LMPD Database

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LMP006842

UniProt Annotations

Entry Information
Gene NameCTP synthase URA7
Protein EntryURA7_YEAST
UniProt IDP28274
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=60 uM for UTP {ECO:0000269|PubMed:8075080}; KM=0.9 mM for ATP {ECO:0000269|PubMed:8075080}; KM=0.4 mM for L-glutamine {ECO:0000269|PubMed:8075080}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:8075080};
Biophysicochemical PropertiesKinetic parameters: KM=60 uM for UTP ; KM=0.9 mM for ATP ; KM=0.4 mM for L-glutamine ; pH dependence: Optimum pH is 8.0. ;
Catalytic ActivityATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
Enzyme RegulationActivated by GTP and inhibited by CTP
Enzyme RegulationActivated by GTP and inhibited by CTP. {ECO:0000269|PubMed:8075080}.
FunctionCatalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.
InteractionQ03690:TIF31; NbExp=3; IntAct=EBI-20128, EBI-8989;
MiscellaneousPresent with 57600 molecules/cell in log phase SD medium
MiscellaneousPresent with 57600 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayPyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.
SimilarityBelongs to the CTP synthase family
SimilarityBelongs to the CTP synthase family. {ECO:0000305}.
SimilarityContains 1 glutamine amidotransferase type-1 domain
SimilarityContains 1 glutamine amidotransferase type-1 domain. {ECO:0000255|PROSITE-ProRule:PRU00605}.
SubunitHomodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP
SubunitHomodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP. {ECO:0000269|PubMed:8075080}.