LMPD Database

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LMP006863

UniProt Annotations

Entry Information
Gene NameCTP synthase URA8
Protein EntryURA8_YEAST
UniProt IDP38627
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=74 uM for UTP {ECO:0000269|PubMed:7559626}; KM=22 uM for ATP {ECO:0000269|PubMed:7559626}; KM=0.14 mM for L-glutamine {ECO:0000269|PubMed:7559626}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:7559626};
Biophysicochemical PropertiesKinetic parameters: KM=74 uM for UTP ; KM=22 uM for ATP ; KM=0.14 mM for L-glutamine ; pH dependence: Optimum pH is 7.5. ;
Catalytic ActivityATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420;
Enzyme RegulationActivated by GTP. Subject to allosteric product inhibition by CTP. Inhibited by p-chloromercuriphenylsulfonic acid, N-ethylmaleimide and cyclopentenylcytosine (CPEC)
Enzyme RegulationActivated by GTP. Subject to allosteric product inhibition by CTP. Inhibited by p-chloromercuriphenylsulfonic acid, N-ethylmaleimide and cyclopentenylcytosine (CPEC). {ECO:0000269|PubMed:7559626, ECO:0000269|PubMed:9668079}.
FunctionCatalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Plays an important role in the regulation of phospholipid synthesis.
MiscellaneousPresent with 5370 molecules/cell in log phase SD medium
MiscellaneousPresent with 5370 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayPyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.
Sequence CautionSequence=CAA89633.1; Type=Frameshift; Positions=559; Evidence= ;
Sequence CautionSequence=CAA89633.1; Type=Frameshift; Positions=559; Evidence={ECO:0000305};
SimilarityBelongs to the CTP synthase family
SimilarityBelongs to the CTP synthase family. {ECO:0000305}.
SimilarityContains 1 glutamine amidotransferase type-1 domain
SimilarityContains 1 glutamine amidotransferase type-1 domain. {ECO:0000255|PROSITE-ProRule:PRU00605}.
Subcellular LocationCytoplasm .
Subcellular LocationCytoplasm {ECO:0000269|PubMed:14562095}.
SubunitHomodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP
SubunitHomodimer. Oligomerizes to a tetramer in the presence of its substrates UTP and ATP. {ECO:0000269|PubMed:7559626}.