LMPD Database

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LMP007050

UniProt Annotations

Entry Information
Gene Namebifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
Protein EntryEPT1_YEAST
UniProt IDP22140
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=120 uM for CDP-choline {ECO:0000269|PubMed:1847919}; KM=29 uM for CDP-dimethylethanolamine {ECO:0000269|PubMed:1847919}; KM=29 uM for CDP-monomethylethanolamine {ECO:0000269|PubMed:1847919}; KM=22 uM for CDP-ethanolamine {ECO:0000269|PubMed:1847919}; Vmax=0.62 nmol/min/mg enzyme for CDP-choline {ECO:0000269|PubMed:1847919}; Vmax=0.42 nmol/min/mg enzyme for CDP-dimethylethanolamine {ECO:0000269|PubMed:1847919}; Vmax=0.27 nmol/min/mg enzyme for CDP-monomethylethanolamine {ECO:0000269|PubMed:1847919}; Vmax=1.35 nmol/min/mg enzyme for CDP-ehylethanolamine {ECO:0000269|PubMed:1847919};
Catalytic ActivityCDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.
Catalytic ActivityCDP-ethanolamine + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylethanolamine.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:1847919};
Enzyme RegulationRequires a divalent cation activator, and is inhibited by CMP. Activated by phospholipids, especially phosphatidylcholine. {ECO:0000269|PubMed:1847919}.
FunctionCatalyzes the final step in the CDP-ethanolamine route leading to phosphatidylethanolamine (PE). Can also catalyze the formation of phosphatidylcholine (PC) from CDP-choline, but does not substantially contribute to PC biosynthesis. Preferentially uses CDP-dimethylethanolamine and CDP-propanolamine as aminoalcohol substrates. Shows highest activity toward di- unsaturated diacylglycerol species as lipid substrates. The CDP- ethanolamine pathway may play a role in maintaining the proper PE species distribution. {ECO:0000269|PubMed:15225629, ECO:0000269|PubMed:1847919, ECO:0000269|PubMed:2848840, ECO:0000269|PubMed:7961445, ECO:0000269|PubMed:7961735}.
InductionRepressed by inositol. Repression is dependent on the presence of CPT1. {ECO:0000269|PubMed:7961831}.
PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.
PathwayPhospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.
SimilarityBelongs to the CDP-alcohol phosphatidyltransferase class-I family. {ECO:0000305}.
Subcellular LocationGolgi apparatus membrane; Multi-pass membrane protein.