LMPD Database

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LMP007094

UniProt Annotations

Entry Information
Gene Namebifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Protein EntryPEM2_YEAST
UniProt IDP05375
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=54 uM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; KM=59 uM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; KM=380 uM for phosphatidyl-N-methylethanolamine (PMME) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; KM=180 uM for phosphatidyl-N-dimethylethanolamine (PDME) {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666}; pH dependence: Optimum pH is 8.1. {ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:2684666};
Catalytic ActivityS-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine. {ECO:0000255|PROSITE- ProRule:PRU00931}.
Catalytic ActivityS-adenosyl-L-methionine + phosphatidyl-N- dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine. {ECO:0000255|PROSITE-ProRule:PRU00931}.
Catalytic ActivityS-adenosyl-L-methionine + phosphatidyl-N- methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N- dimethylethanolamine. {ECO:0000255|PROSITE-ProRule:PRU00931}.
FunctionCatalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC). {ECO:0000255|PROSITE-ProRule:PRU00931, ECO:0000269|PubMed:2198947, ECO:0000269|PubMed:22001639, ECO:0000269|PubMed:2445736, ECO:0000269|PubMed:2670666, ECO:0000269|PubMed:2684666, ECO:0000269|PubMed:2850468, ECO:0000269|PubMed:6337128, ECO:0000269|PubMed:7047296}.
InductionExpression is repressed by inositol and choline. The 5' flanking region contains two copies of the CATRTGAA motif and a 5'-AAACCCACACATG-3' GRFI site, which are involved in the regulation of expression. OPI1 and SIN3 play the role of repressors for OPI3 expression whereas UME6 is an activator of OPI3 expression. {ECO:0000269|PubMed:1650774, ECO:0000269|PubMed:1954254, ECO:0000269|PubMed:6759124, ECO:0000269|PubMed:8614637}.
MiscellaneousPresent with 5890 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis. {ECO:0000255|PROSITE-ProRule:PRU00931}.
SimilarityBelongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU00931}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.