LMPD Database

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LMP007195

UniProt Annotations

Entry Information
Gene NameDfg10p
Protein EntryDFG10_YEAST
UniProt IDP40526
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityDitrans,polycis-dolichol + NADP(+) = ditrans,polycis-polyprenol + NADPH. {ECO:0000269|PubMed:20637498}.
Disruption PhenotypeSuppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N- glycosylation. {ECO:0000269|PubMed:20637498, ECO:0000269|PubMed:9055077}.
FunctionPlays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. {ECO:0000269|PubMed:20637498}.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.