LMPD Database

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LMP007253

UniProt Annotations

Entry Information
Gene Namesphinganine kinase LCB4
Protein EntryLCB4_YEAST
UniProt IDQ12246
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=7.7 uM for dihydrosphingosine {ECO:0000269|PubMed:9677363}; KM=25 uM for ATP {ECO:0000269|PubMed:9677363};
Catalytic ActivityATP + phytosphingosine = ADP + phytosphingosine 1-phosphate.
Catalytic ActivityATP + sphinganine = ADP + sphinganine 1- phosphate.
FunctionCatalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Involved in the biosynthesis of sphingolipids and ceramides. Required with LCB3 for an effective incorporation of DHS into ceramides through a phosphorylation-dephosphorylation cycle. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance. {ECO:0000269|PubMed:11056159, ECO:0000269|PubMed:11102354, ECO:0000269|PubMed:11278643, ECO:0000269|PubMed:11795842, ECO:0000269|PubMed:11967828, ECO:0000269|PubMed:12493772, ECO:0000269|PubMed:16141212, ECO:0000269|PubMed:9677363}.
MiscellaneousPresent with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PtmPhosphorylated by the cyclin-CDKs PCL1-PHO85 and PCL2-PHO85. Phosphorylation is a prerequisite to ubiquitination. The phosphorylation level depends on sterol composition and may also be involved in subcellular location. {ECO:0000269|PubMed:15598647, ECO:0000269|PubMed:16141212, ECO:0000269|PubMed:17330950, ECO:0000269|PubMed:18407956, ECO:0000269|PubMed:19779198}.
PtmUbiquitinated. The ubiquitination leads to degradation in the vacuole. {ECO:0000269|PubMed:15598647}.
SimilarityContains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.
Subcellular LocationCell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein.