LMPD Database

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LMP007362

UniProt Annotations

Entry Information
Gene Name3-keto-steroid reductase
Protein EntryERG27_YEAST
UniProt IDQ12452
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityA 3-beta-hydroxysteroid + NADP(+) = a 3- oxosteroid + NADPH.
FunctionResponsible for the reduction of the keto group on the C-3 of sterols. Also facilitates the association of ERG7 with lipid particles preventing its digestion in the endoplasmic reticulum and the lipid particles. {ECO:0000269|PubMed:10535978, ECO:0000269|PubMed:12842197}.
InteractionP53045:ERG25; NbExp=4; IntAct=EBI-38132, EBI-6506; P40030:ERG28; NbExp=6; IntAct=EBI-38132, EBI-22518; P38604:ERG7; NbExp=4; IntAct=EBI-38132, EBI-6572;
PathwaySteroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
SimilarityBelongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane; Peripheral membrane protein. Lipid droplet.
SubunitHeterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation- related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG25 and ERG28. Also interacts with ERG7, but only in lipid particles. {ECO:0000269|PubMed:12119386, ECO:0000269|PubMed:12842197, ECO:0000269|PubMed:15995173}.