LMPD Database

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LMP007413

UniProt Annotations

Entry Information
Gene NameMcr1p
Protein EntryMCR1_YEAST
UniProt IDP36060
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityNADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5.
CofactorName=FAD; Xref=ChEBI:CHEBI:57692;
FunctionThe outer membrane form may mediate the reduction of outer membrane cytochrome b5, and the soluble inter-membrane space form may transfer electrons from external NADH to cytochrome c, thereby mediating an antimycin-insensitive, energy-coupled oxidation of external NADH by yeast mitochondria. Involved in the reduction of D-erythroascorbyl free radicals
FunctionThe outer membrane form may mediate the reduction of outer membrane cytochrome b5, and the soluble inter-membrane space form may transfer electrons from external NADH to cytochrome c, thereby mediating an antimycin-insensitive, energy-coupled oxidation of external NADH by yeast mitochondria. Involved in the reduction of D-erythroascorbyl free radicals. {ECO:0000269|PubMed:11420140}.
InductionBy osmotic stress
InductionBy osmotic stress. {ECO:0000269|PubMed:15590813}.
InteractionP39940:RSP5; NbExp=2; IntAct=EBI-10565, EBI-16219;
Mass SpectrometryMass=34137; Method=Unknown; Range=1-302; Evidence= ;
Mass SpectrometryMass=34137; Method=Unknown; Range=1-302; Evidence={ECO:0000269|PubMed:16689936};
MiscellaneousPresent with 1920 molecules/cell in log phase SD medium
MiscellaneousPresent with 1920 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PtmThere are two isoforms of NADH-cytochrome b5 reductase, a 34 kDa form (p34) and a 32 kDa form (p32). The p34 form becomes firmly anchored to the outer mitochondrial membrane after an incomplete translocation arrest. The p32 form is formed after translocation of the p34 precursor to the inner mitochondrial membrane, where it is processed by mitochondrial inner membrane peptidase (IMP) complex and released to the intermembrane space.
SimilarityBelongs to the flavoprotein pyridine nucleotide cytochrome reductase family
SimilarityBelongs to the flavoprotein pyridine nucleotide cytochrome reductase family. {ECO:0000305}.
SimilarityContains 1 FAD-binding FR-type domain
SimilarityContains 1 FAD-binding FR-type domain. {ECO:0000255|PROSITE-ProRule:PRU00716}.
Subcellular LocationNADH-cytochrome b5 reductase p32 form: Mitochondrion intermembrane space.
Subcellular LocationNADH-cytochrome b5 reductase p34 form: Mitochondrion outer membrane; Single-pass membrane protein.