LMPD Database

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LMP007444

UniProt Annotations

Entry Information
Gene Namebranched-chain-amino-acid transaminase BAT2
Protein EntryBCA2_YEAST
UniProt IDP47176
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityL-isoleucine + 2-oxoglutarate = (S)-3-methyl- 2-oxopentanoate + L-glutamate.
Catalytic ActivityL-leucine + 2-oxoglutarate = 4-methyl-2- oxopentanoate + L-glutamate.
Catalytic ActivityL-valine + 2-oxoglutarate = 3-methyl-2- oxobutanoate + L-glutamate.
CofactorName=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
Developmental StageHighly expressed during stationary phase, down-regulated during logarithmic phase of growth.
FunctionCatalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using branched chain amino acids (leucine, isoleucine, and valine) as well as lysine and proline as the amino donors. Involved in cell cycle regulation. {ECO:0000269|PubMed:18625006, ECO:0000269|PubMed:21267457}.
InductionDown-regulated in the presence of repressive nitrogen sources (glutamine) and derepressed in secondary non-repressive nitrogen sources such as GABA. Highly expressed on cultures with isoleucine, leucine and valine as sole nitrogen source (catabolic conditions). {ECO:0000269|PubMed:21267457}.
InteractionP22696:ESS1; NbExp=1; IntAct=EBI-3462, EBI-6679;
MiscellaneousPresent with 25900 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayAmino-acid biosynthesis; L-isoleucine biosynthesis; L- isoleucine from 2-oxobutanoate: step 4/4.
PathwayAmino-acid biosynthesis; L-leucine biosynthesis; L- leucine from 3-methyl-2-oxobutanoate: step 4/4.
PathwayAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.
PathwayAmino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.
Sequence CautionSequence=CAA60376.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA60376.1; Type=Frameshift; Positions=1, 170, 199, 207, 210, 214; Evidence={ECO:0000305};
SimilarityBelongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:21267457}.