LMPD Database

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LMP007548

UniProt Annotations

Entry Information
Gene Nameglycerol-3-phosphate O-acyltransferase
Protein EntryPLSB_ECOLI
UniProt IDP0A7A7
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=49 uM for glycerol-3-phosphate {ECO:0000269|PubMed:10231527, ECO:0000269|PubMed:7026564}; Vmax=13.1 nmol/min/mg enzyme with glycerol-3-phosphate as substrate {ECO:0000269|PubMed:10231527, ECO:0000269|PubMed:7026564}; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:10231527, ECO:0000269|PubMed:7026564};
Catalytic ActivityAcyl-CoA + sn-glycerol 3-phosphate = CoA + 1- acyl-sn-glycerol 3-phosphate.
DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
FunctionCatalyzes the transfer of an acyl group from acyl-ACP to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme can utilize either acyl-CoA or acyl-ACP as the fatty acyl donor. {ECO:0000269|PubMed:17645809, ECO:0000269|PubMed:6997313}.
InteractionP0A8Z3:ybgC; NbExp=3; IntAct=EBI-542961, EBI-543276;
PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Sequence CautionSequence=AAC43135.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
SimilarityBelongs to the GPAT/DAPAT family. {ECO:0000305}.
Subcellular LocationCell inner membrane {ECO:0000269|PubMed:16294310, ECO:0000269|PubMed:4943977}; Peripheral membrane protein {ECO:0000269|PubMed:16294310, ECO:0000269|PubMed:4943977}; Cytoplasmic side {ECO:0000269|PubMed:16294310, ECO:0000269|PubMed:4943977}.
SubunitInteracts with ACP, YbgC and PssA, forming altogether a complex at the inner membrane. {ECO:0000269|PubMed:16294310}.