LMPD Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
LMP007606

UniProt Annotations

Entry Information
Gene Namelipoate synthase
Protein EntryLIPA_ECOLI
UniProt IDP60716
SpeciesE. coli
Comments
Comment typeDescription
Catalytic ActivityProtein N(6)-(octanoyl)lysine + 2 sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)- (lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'- deoxyadenosine. {ECO:0000269|PubMed:15157071}.
CofactorName=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:15362861}; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. {ECO:0000269|PubMed:15362861};
Enzyme RegulationInhibited by the AdoMet analog S-adenosyl homocysteine.
FunctionCatalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Free octanoate is not a substrate for LipA. {ECO:0000269|PubMed:11106496, ECO:0000269|PubMed:14700636, ECO:0000269|PubMed:15157071}.
MiscellaneousThe source of sulfurs is the LipA protein itself, most likely one 4Fe-4S cluster.
PathwayProtein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. {ECO:0000269|PubMed:15112987}.
Sequence CautionSequence=AAA24072.1; Type=Erroneous initiation; Evidence={ECO:0000305};
SimilarityBelongs to the radical SAM superfamily. Lipoyl synthase family. {ECO:0000305}.
Subcellular LocationCytoplasm.
SubunitMonomer or homodimer.