LMPD Database

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LMP007717

UniProt Annotations

Entry Information
Gene Namefused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase
Protein EntryFADB_ECOLI
UniProt IDP21177
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=53 uM for crotonyl-CoA (for enoyl-CoA hydratase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=8.7 mM for L-3-hydroxy-4-trans-decenoyl-CoA (for enoyl-CoA hydratase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=38 mM for D-3-hydroxy-4-trans-decenoyl-CoA (for enoyl-CoA hydratase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=5.8 uM for 3-cis-tetradecenoyl-CoA (for Delta(3)-cis- Delta(2)-trans-enoyl-CoA isomerase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=69 uM for acetoacetyl-CoA (for 3-hydroxyacyl-CoA dehydrogenase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=2.0 uM for NADH (for 3-hydroxyacyl-CoA dehydrogenase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745};
Catalytic Activity(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O. {ECO:0000255|HAMAP-Rule:MF_01621}.
Catalytic Activity(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CoA. {ECO:0000255|HAMAP-Rule:MF_01621}.
Catalytic Activity(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH. {ECO:0000255|HAMAP-Rule:MF_01621}.
Catalytic Activity(S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA. {ECO:0000255|HAMAP-Rule:MF_01621}.
FunctionInvolved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate. {ECO:0000255|HAMAP-Rule:MF_01621, ECO:0000269|PubMed:12535077, ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:368024, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}.
InductionRepressed by FadR in the absence of LCFAs (fatty acids of, at least, 12 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs which bind to FadR resulting in its release from the DNA and thus derepression of the transcription.
PathwayLipid metabolism; fatty acid beta-oxidation. {ECO:0000255|HAMAP-Rule:MF_01621}.
SimilarityIn the C-terminal section; belongs to the 3- hydroxyacyl-CoA dehydrogenase family. {ECO:0000255|HAMAP- Rule:MF_01621}.
SimilarityIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000255|HAMAP-Rule:MF_01621}.
SubunitHeterotetramer of two alpha chains (FadB) and two beta chains (FadA). {ECO:0000255|HAMAP-Rule:MF_01621, ECO:0000269|PubMed:368024}.