LMPD Database

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LMP008852

UniProt Annotations

Entry Information
Gene NameCalcium activated protein for secretion
Protein EntryCAPS_DROME
UniProt IDQ9NHE5
SpeciesDrosophila
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=7; Name=1; IsoId=Q9NHE5-1; Sequence=Displayed; Name=2; Synonyms=D; IsoId=Q9NHE5-2; Sequence=VSP_047915, VSP_047917; Name=3; Synonyms=C; IsoId=Q9NHE5-3; Sequence=VSP_047912, VSP_047915, VSP_047917, VSP_047918, VSP_047920; Note=Incomplete sequence.; Name=4; Synonyms=B; IsoId=Q9NHE5-4; Sequence=VSP_047914; Note=No experimental confirmation available.; Name=5; Synonyms=A; IsoId=Q9NHE5-5; Sequence=VSP_047913, VSP_047915, VSP_047917; Note=No experimental confirmation available.; Name=6; Synonyms=E; IsoId=Q9NHE5-6; Sequence=VSP_047915, VSP_047916, VSP_047917, VSP_047919; Note=No experimental confirmation available.; Name=7; Synonyms=F; IsoId=Q9NHE5-7; Sequence=VSP_047915, VSP_047919; Note=No experimental confirmation available.;
Developmental StageNot detected in early embryos through stage 8. Strongly expressed in neural-specific cells at the onset of early stages of neuronal differentiation beginning at stage 9-10. Accumulates from stage 12 through the rest of embryogenesis in all neuronal cells within the brain and ventral nerve cord (VNC). Remains restricted to the central nervous system and not detected in other tissues at any stage of development. {ECO:0000269|PubMed:11516399}.
Disruption PhenotypeFlies display locomotory deficits and complete embryonic lethality. The mutant NMJ reveals a 50% loss in evoked glutamatergic transmission, and an accumulation of synaptic vesicles at active zones. They also display a 3-fold accumulation of DCVs in synaptic terminals. {ECO:0000269|PubMed:11516399}.
DomainThe PH domain is essential for regulated exocytosis and binds phospholipids. {ECO:0000250}.
FunctionCalcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles. However, it probably also participates in small clear synaptic vesicles (SVs) exocytosis and it is unclear whether its function is related to Ca(2+) triggering. {ECO:0000269|PubMed:11516399}.
Sequence CautionSequence=AAD38612.1; Type=Miscellaneous discrepancy; Evidence={ECO:0000305}; Sequence=AAF34697.1; Type=Frameshift; Positions=1283, 1411; Evidence={ECO:0000305}; Sequence=AAM48456.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityContains 1 C2 domain. {ECO:0000305}.
SimilarityContains 1 MHD1 (MUNC13 homology domain 1) domain. {ECO:0000255|PROSITE-ProRule:PRU00587}.
SimilarityContains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}.
Subcellular LocationCytoplasmic vesicle membrane {ECO:0000269|PubMed:11516399}. Cell junction, synapse {ECO:0000269|PubMed:11516399}. Note=Membrane-associated to vesicles. Restricted to all classes of presynaptic termini independent of the ratio of vesicular content (DCVs versus SVs). Found in all identified classes of peripheral and central synapses. In the CNS, it is highly enriched in the synapse-dense neuropil, which lacks cell bodies. Present in all synapses in the neuropil where it precisely colocalizes with other pansynaptic markers. Similarly abundant in all classes of neuromuscular junction (NMJ) termini, including type I, II, and III NMJs, where it localizes to all 3 bouton types. Expressed at similar levels in boutons which contain primarily small clear glutamatergic vesicles, as well as boutons very highly enriched in large DCVs. It appears that neither bouton class contains a pure population of either SVs or DCVs, but only differs dramatically in the relative abundance of the vesicular classes. Thus, the level of expression does not correlate with the abundance of DCVs. Clearly present presynaptically and colocalizes exclusively with presynaptic markers.
Tissue SpecificityRestricted to the nervous system at all stages of development and highly localized at synapses (at protein level). {ECO:0000269|PubMed:11516399}.