LMPD Database

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LMP009687

UniProt Annotations

Entry Information
Gene Name3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Protein EntryHMDH1_ARATH
UniProt IDP14891
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative initiation; Named isoforms=2; Name=Short; Synonyms=HMGR1S; IsoId=P14891-1; Sequence=Displayed; Name=Long; Synonyms=HMGR1L; IsoId=P14891-2; Sequence=VSP_041282;
Catalytic Activity(R)-mevalonate + CoA + 2 NADP(+) = (S)-3- hydroxy-3-methylglutaryl-CoA + 2 NADPH. {ECO:0000255|PROSITE- ProRule:PRU10003}.
Disruption PhenotypeDwarfing, early senescence, and sterility. 65% lower levels in triterpenoids content and 50% lower levels in sterol content. Hmg1 and hmg2 double mutants are lethal during male gametophyte development. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19363204}.
DomainThe N-terminal domain (1-178) of the short isoform is necessary and sufficient for directing the protein to the endoplasmic reticulum and to spherical structures.
Enzyme RegulationRegulated at the post-translational level in response to alterations of sphingolipid and sterol biosynthetic pathways. Negatively regulated by a PP2A-dependent dephosphorylation occurring at a site different than Ser-577. Completely inhibited by mevinolin (IC(50) = 12.5 nM). {ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:21478440, ECO:0000269|PubMed:23404890, ECO:0000269|PubMed:7588795}.
FunctionCatalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:19363204}.
InductionDown-regulated by light in immature leaves, but not in roots. Not regulated by myriocin, squalestatin or terbinafine. {ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:8742338, ECO:0000269|PubMed:9107038}.
PathwayMetabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
PtmReversibly inactivated by phosphorylation at Ser-577 by spinach or Brassica oleracea HMGR kinases in a cell-free system. Probably also phosphorylated at additional sites. {ECO:0000269|PubMed:10318703, ECO:0000269|PubMed:7588795}.
SimilarityBelongs to the HMG-CoA reductase family. {ECO:0000305}.
Subcellular LocationIsoform Long: Endoplasmic reticulum membrane; Multi-pass membrane protein.
Subcellular LocationIsoform Short: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=also observed within spherical structures located along the endoplasmic reticulum strands.
SubunitInteracts (via N-terminus) with B''ALPHA and B''BETA. {ECO:0000269|PubMed:21478440}.
Tissue SpecificityFound in all tissues. Isoform Short is expressed at low levels specifically in flowers. Expressed in both the tapetum and microspores. {ECO:0000269|PubMed:19363204, ECO:0000269|PubMed:7496400, ECO:0000269|PubMed:8302869}.