LMPD Database

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LMP009711

UniProt Annotations

Entry Information
Gene Name3-ketoacyl-CoA thiolase 2
Protein EntryTHIK2_ARATH
UniProt IDQ56WD9
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=77.5 uM for acetoacetyl-CoA (at 25 degrees Celsius) {ECO:0000269|PubMed:11696182}; Vmax=5000 nmol/min/mg enzyme (at 25 degrees Celsius) {ECO:0000269|PubMed:11696182};
Catalytic ActivityAcyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
Developmental StageLevels increase strongly upon imbibition and decrease 3 days later. Strongly induced in leaves during senescence. {ECO:0000269|PubMed:11356168, ECO:0000269|PubMed:11696182, ECO:0000269|PubMed:11891244}.
FunctionInvolved in long chain fatty-acid beta-oxidation prior to gluconeogenesis during germination and subsequent seedling growth. Confers sensitivity to 2,4-dichlorophenoxybutiric acid (2,4-DB). Required for local and systemic induction of jasmonic acid (JA) biosynthesis after wounding. Seems to be involved in JA biosynthesis during senescence. {ECO:0000269|PubMed:11696182, ECO:0000269|PubMed:11891244, ECO:0000269|PubMed:15141068, ECO:0000269|PubMed:15979881, ECO:0000269|PubMed:9490742}.
InductionExpressed in the dark, repressed by light (at protein level). Induced by dehydration, by abscisic acid (ABA) and by wounding, both locally and systemically. {ECO:0000269|PubMed:10212254, ECO:0000269|PubMed:15141068, ECO:0000269|PubMed:9490742}.
PathwayLipid metabolism; fatty acid metabolism.
Sequence CautionSequence=AAL25590.1; Type=Frameshift; Positions=375; Evidence={ECO:0000305};
SimilarityBelongs to the thiolase family. {ECO:0000305}.
Subcellular LocationPeroxisome. Glyoxysome. Note=Peroxisomal targeting signal type 2 (PTS2-type) import is temperature and ATP- dependent, induced by zinc ions and PTS1-type import, but repressed by mature forms of PED1.
SubunitHomodimer. {ECO:0000269|PubMed:16630629}.
Tissue SpecificityAccumulates in etiolated cotyledons and in seedlings, also present in roots, flowers and siliques (at protein level). High levels in wounded leaves. {ECO:0000269|PubMed:10212254, ECO:0000269|PubMed:11696182, ECO:0000269|PubMed:15141068, ECO:0000269|PubMed:9490742}.