LMPD Database

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LMP009721

UniProt Annotations

Entry Information
Gene Name3-oxoacyl-[acyl-carrier-protein] synthase
Protein EntryKASM_ARATH
UniProt IDQ8L3X9
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityAcyl-[acyl-carrier-protein] + malonyl-[acyl- carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein].
Disruption PhenotypeSlow growth and bleached leaf phenotype when grown under ambient air, but normal growth under CO(2)-enriched air. Highly prevents lipoylation of the H-protein subunit of the glycine decarboxylase (GDC) in leaves, but has only a limited effect on the lipoylation of the E2 subunits of pyruvate dehydrogenase (PDH) and alpha-ketoacid dehydrogenase (KGDH) complexes in leaves and even no effect in roots. {ECO:0000269|PubMed:17616510}.
Enzyme RegulationInhibited by cerulenin.
FunctionCatalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins. {ECO:0000269|PubMed:17616510}.
MiscellaneousMitochondrial protein lipoylation in leaves does not exclusively depend on the lipoate biosynthesis by KAS and may occur independently of this pathway in roots. {ECO:0000305|PubMed:17616510}.
PathwayLipid metabolism; fatty acid biosynthesis.
Sequence CautionSequence=AAD25826.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the beta-ketoacyl-ACP synthases family. {ECO:0000305}.
Subcellular LocationMitochondrion {ECO:0000269|PubMed:14660674}.
SubunitHomodimer. {ECO:0000269|PubMed:15527780}.
Tissue SpecificityExpressed at the same level in leaves, roots and flowers. {ECO:0000269|PubMed:14660674}.