LMPD Database

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LMP009842

UniProt Annotations

Entry Information
Gene Namealpha-dioxygenase
Protein EntryDOX1_ARATH
UniProt IDQ9SGH6
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=18 uM for linolenic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; KM=17 uM for linoleic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; KM=13 uM for oleic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; KM=11 uM for palmitic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=119 nmol/min/mg enzyme with linolenic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=111 nmol/min/mg enzyme with linoleic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=91 nmol/min/mg enzyme with oleic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=46 nmol/min/mg enzyme with palmitic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113};
Disruption PhenotypeSlightly higher accumulation of Pseudomonas syringae pv. tomato DC3000 in infected leaves. Partially impaired systemic acquired resistance (SAR) in response to incompatible interaction. When associated with LOX1 disruption; hypersensitivity to the growth-inhibitory effect of abscisic acid (ABA) accompanied by an accumulation of ABA-inducible marker genes. {ECO:0000269|PubMed:22199234}.
FunctionAlpha-dioxygenase that catalyzes the primary oxygenation of fatty acids into oxylipins. Mediates a protection against oxidative stress and cell death, probably by generating some lipid-derived molecules. Promotes local and systemic plant defense in a salicylic acid (SA)-dependent manner, including the establishment of systemic acquired resistance (SAR) in response to incompatible interaction. Involved in a negative regulation of abscisic acid (ABA)-mediated signaling pathway. {ECO:0000269|PubMed:10455113, ECO:0000269|PubMed:12060227, ECO:0000269|PubMed:22199234}.
InductionInduced by salicylic acid (SA) and by some chemicals generating reactive oxygen species (ROS, e.g. nitric oxide (NO), intracellular superoxide and singlet oxygen) such as sodium nitropruside (SNP), paraquat and rose bengal (RB). Accumulates locally, at the infection site, in response to both incompatible and compatible bacterial pathogens (e.g. Pseudomonas syringae pv. tomato DC3000) in a SA-dependent manner, with a faster response during hypersensitive reactions. {ECO:0000269|PubMed:12060227}.
SimilarityBelongs to the peroxidase family. {ECO:0000255|PROSITE-ProRule:PRU00298}.
Tissue SpecificityExpressed in roots (epiderm), mature flowers (e.g. anthers) and senescing leaves. {ECO:0000269|PubMed:12060227, ECO:0000269|PubMed:17369372}.