LMPD Database

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LMP009978

UniProt Annotations

Entry Information
Gene Namelysophospholipase 2
Protein EntryCSE_ARATH
UniProt IDQ9C942
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=96.5 uM for caffeoylshikimate {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498}; KM=211 uM for 4-coumarylshikimate {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498}; KM=6.6 uM for lysophosphatidylcholine (at pH 8.0 and 33 degrees Celsius) {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498}; Vmax=9.3 pmol/sec/mg enzyme toward caffeoylshikimate {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498}; Vmax=0.66 pmol/sec/mg enzyme toward 4-coumarylshikimate {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498}; Vmax=0.030 umol/min/mg enzyme toward lysophosphatidylcholine (at pH 8.0 and 33 degrees Celsius) {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498};
Catalytic ActivityCaffeoylshikimate + H(2)O = caffeate + shikimate. {ECO:0000269|PubMed:23950498}.
Disruption PhenotypeReduced height and weight of senescent plants due to reduced lignin content. {ECO:0000269|PubMed:23950498}.
FunctionEsterase involved in the biosynthesis of lignin. Hydrolyzes caffeoylshikimate into caffeate and shikimate. Together with 4-coumarate--CoA ligase (4CL), acts on an alternative reaction for the formation of caffeoyl-CoA and bypasses the second reaction of shikimate O-hydroxycinnamoyltransferase (HST). Accepts also 4-coumaroylshikimate as substrate, but with lower activity. According to PubMed:20345607 and PubMed:22915575, posseses monoacylglycerol O-acyltransferase, monoacylglycerol lipase and lysophospholipase activities in vitro. With the association of ACBP2, may promote the degradation of lysophosphatidylcholine and detoxify the peroxidized membrane in response to cadmium-induced oxidative stress. However these results require additional confirmation in vivo. {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:20657176, ECO:0000269|PubMed:22915575, ECO:0000269|PubMed:23950498}.
InductionBy zinc and H(2)O(2). {ECO:0000269|PubMed:20345607}.
MiscellaneousMutant plants exhibit increased sensitivity to zinc, cadmium and H(2)O(2). {ECO:0000305|PubMed:20345607}.
SimilarityBelongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. {ECO:0000305}.
Subcellular LocationCell membrane {ECO:0000305|PubMed:20345607}; Peripheral membrane protein {ECO:0000305|PubMed:20345607}. Note=Colocalizes with ACBP2.
SubunitInteracts with ACBP2. {ECO:0000269|PubMed:20345607}.
Tissue SpecificityExpressed in vasculature of roots and leaves, stems, flowers and siliques. {ECO:0000269|PubMed:20345607, ECO:0000269|PubMed:23950498}.