LMPD Database

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LMP010135

UniProt Annotations

Entry Information
Gene Namediacylglycerol O-acyltransferase 1
Protein EntryDGAT1_ARATH
UniProt IDQ9SLD2
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiespH dependence: Optimum pH is between 6.75-7.25. {ECO:0000269|PubMed:11171171};
Catalytic ActivityAcyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. {ECO:0000269|PubMed:11171171}.
Developmental StagePeak of expression in seeds 15 days after flowering. Highly expressed in senescing leaves. {ECO:0000269|PubMed:12177474, ECO:0000269|PubMed:20101470}.
Disruption PhenotypeDecreased oil content and modified size and shape of oil bodies. {ECO:0000269|PubMed:20040537}.
Enzyme RegulationPartially inhibited by niacin. {ECO:0000269|PubMed:11171171}.
FunctionMajor enzyme for oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen. {ECO:0000269|PubMed:10580283, ECO:0000269|PubMed:10601854, ECO:0000269|PubMed:12114588, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}.
InductionUp-regulated by glucose. {ECO:0000269|PubMed:12825687}.
MiscellaneousDGAT1 deficiency alters carbohydrate metabolism.
MiscellaneousThe AS11 mutant has a complete duplication of exon 2.
PathwayGlycerolipid metabolism; triacylglycerol biosynthesis.
SimilarityBelongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. {ECO:0000305}.
Subcellular LocationPlastid, chloroplast membrane {ECO:0000269|PubMed:12177474}; Multi-pass membrane protein {ECO:0000269|PubMed:12177474}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:12177474}; Multi-pass membrane protein {ECO:0000269|PubMed:12177474}.
Tissue SpecificityUbiquitous. Highest expression in young developing seeds. {ECO:0000269|PubMed:10571850, ECO:0000269|PubMed:12825687, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}.