LMPD Database

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LMP010728

UniProt Annotations

Entry Information
Gene Namefatty acyl-ACP thioesterases B
Protein EntryFATB_ARATH
UniProt IDQ9SJE2
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=2.9 uM for myristoyl-ACP (14:0-ACP) {ECO:0000269|PubMed:12061798}; KM=4.0 uM for myristoleoyl-ACP (14:1-ACP) {ECO:0000269|PubMed:12061798}; KM=3.8 uM for palmitoyl-ACP (16:0-ACP) {ECO:0000269|PubMed:12061798}; KM=2.6 uM for palmitoleoyl-ACP (16:1-ACP) {ECO:0000269|PubMed:12061798}; KM=3.0 uM for stearoyl-ACP (18:0-ACP) {ECO:0000269|PubMed:12061798}; KM=2.6 uM for oleoyl-ACP (18:1-ACP) {ECO:0000269|PubMed:12061798}; Note=The catalytic efficiency for 16:0 is at least 2-fold higher than for other substrates.; pH dependence: Optimum pH is 9.7 {ECO:0000269|PubMed:7840673};
Catalytic ActivityPalmitoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + palmitate. {ECO:0000269|PubMed:12061798, ECO:0000269|PubMed:7840673}.
Disruption PhenotypeRetarded growth, deformed seeds with low rate of germination and reduced levels of palmitate and stearate. {ECO:0000269|PubMed:12671095, ECO:0000269|PubMed:18263779, ECO:0000269|PubMed:18326828}.
FunctionPlays an essential role in chain termination during de novo fatty acid synthesis. Possesses high thioesterase activity for palmitoyl-ACP versus other acyl-ACPs. Substrate preference is 16:0 > 18:1 > 18:0 > 16:1. Plays an essential role in the supply of saturated fatty acids necessary for plant growth and seed development. Contributes to 16:0 production particularly in flowers. May be involved in the synthesis of long chain fatty acid. {ECO:0000269|PubMed:10859193, ECO:0000269|PubMed:12061798, ECO:0000269|PubMed:12671095, ECO:0000269|PubMed:15531590, ECO:0000269|PubMed:18263779, ECO:0000269|PubMed:18326828, ECO:0000269|PubMed:7840673}.
MiscellaneousPlants silencing FATB, show reduced level of palmitate in flowers and seeds. {ECO:0000305|PubMed:10859193}.
SimilarityBelongs to the acyl-ACP thioesterase family. {ECO:0000305}.
Subcellular LocationPlastid, chloroplast {ECO:0000305}.
Tissue SpecificityHighly expressed in flowers. Expressed in roots, leaves, stems, siliques and seeds. {ECO:0000269|PubMed:10859193, ECO:0000269|PubMed:7840673}.