LMPD Database

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LMP010899

UniProt Annotations

Entry Information
Gene Namephospholipid-transporting ATPase 3
Protein EntryALA3_ARATH
UniProt IDQ9XIE6
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).
Disruption PhenotypeImpaired growth of roots and shoots. Roots devoided of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion. Aberrant trichome expansion, reduced primary root growth and longer root hairs. Impaired pollen growth. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:19566596}.
FunctionInvolved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:19566596, ECO:0000269|PubMed:20053675}.
MiscellaneousThe intracellular targeting signals and lipid specificity determinants reside in the catalytic ALA subunit.
Sequence CautionSequence=AAD39325.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. {ECO:0000305}.
Subcellular LocationGolgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Requires the presence of an ALIS protein to exit the endoplasmic reticulum to the Golgi.
SubunitAssociates with ALIS1 to form a stable and active complex. Interacts with ALIS3 and ALIS5 in a heterologous system. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:20053675}.
Tissue SpecificityExpressed in petals and sepals, but not in reproductive tissues. In siliques, detected in the upper part of the seed pod and in the area between the seed pod and the stem, but not in developing seeds. Strong expression in vascular shoot tissues and in stomatal guard cells of young rosettes leaves. In roots, expressed in cells surrounding the xylem and in central and peripheral columella cells. Detected in developing and mature trichomes, roots, pollen and growing pollen tubes. {ECO:0000269|PubMed:18344284, ECO:0000269|PubMed:19566596}.