LMPD Database

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LMP011304

UniProt Annotations

Entry Information
Gene NameDiacylglycerol kinase family protein
Protein EntrySPHK2_ARATH
UniProt IDF2Y4A3
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityATP + sphinganine = ADP + sphinganine 1- phosphate. {ECO:0000269|PubMed:21330371}.
Catalytic ActivityATP + sphingosine = ADP + sphingosine 1- phosphate. {ECO:0000269|PubMed:21330371}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420;
Disruption PhenotypeNo visible phenotype under normal growth conditions. {ECO:0000269|PubMed:22275366}.
Enzyme RegulationActivated by phosphatidic acid (PA). Binding with PA stimulates the activity by promoting the binding of substrate to the catalytic site.
FunctionInvolved in the production of sphingolipid metabolites. Phosphorylates sphingosine and various l sphingoid long-chain base (LCB) products, such as phytosphingosine (PHS, 4- hydroxysphinganine), 4-hydroxy-8-sphingenine, 4,8-sphingadienine and D-erythro-dihydrosphingosine, but has a very few activity toward D,L-threo- dihydrosphingosine. Is required for abscisic acid (ABA) signaling that mediates stomatal closure, inhibition of seed germination and root elongation. May function upstream of PLDALPHA1 and phosphatidic acid (PA) in an amplification response to ABA that mediates stomatal closure. {ECO:0000269|PubMed:21330371, ECO:0000269|PubMed:22275366}.
Sequence CautionSequence=CAA18718.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g21540 has been split into 2 genes: At4g21534 and At4g21540.; Evidence={ECO:0000305}; Sequence=CAB81261.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g21540 has been split into 2 genes: At4g21534 and At4g21540.; Evidence={ECO:0000305};
SimilarityContains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.
Subcellular LocationVacuole membrane {ECO:0000305|PubMed:21330371}; Peripheral membrane protein {ECO:0000305|PubMed:21330371}. Note=Associated with the tonoplast.
Tissue SpecificityHihgly expressed in flowers and siliques and at lower levels in roots, leaves and stems. {ECO:0000269|PubMed:21330371}.