LMPD Database

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LMP011306

UniProt Annotations

Entry Information
Gene Nameprobable squalene epoxidase 1
Protein EntryERG14_ARATH
UniProt IDQ9SM02
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivitySqualene + NADPH + O(2) = (3S)-2,3-epoxy-2,3- dihydrosqualene + NADP(+) + H(2)O. {ECO:0000269|PubMed:17426032}.
CofactorName=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
Disruption PhenotypeNon viable, sterile dwarf plants with short, highly branched roots. {ECO:0000269|PubMed:17426032}.
FunctionCatalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. Can produce not only oxidosqualene, but also 2,3:22,23-dioxidosqualene. Main squalene epoxidase in the root. Sqe1 mutants may show defects in membrane lipid rafts, impairing the correct localization of RHD2 NADPH oxidase and the proper polarized production of ROS. {ECO:0000269|PubMed:17426032, ECO:0000269|PubMed:19309460, ECO:0000269|PubMed:19847116}.
MiscellaneousSEQ4 or SEQ5 are unable to complement seq1 mutants. {ECO:0000305|PubMed:17426032}.
PathwayTerpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.
Sequence CautionSequence=AAG50645.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AEM36345.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the squalene monooxygenase family. {ECO:0000305}.
Subcellular LocationMembrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Tissue SpecificityExpressed in seedlings, leaves, stems, inflorescences, sepals, style and siliques. Expressed in expanded cotyledons, root tips and cortical cells of the root elongation zone, but not in root hair cells. In leaves, expressed in most cells, with a very strong expression in stomata. {ECO:0000269|PubMed:17426032, ECO:0000269|PubMed:19309460}.