LMPD Database

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LMP011315

UniProt Annotations

Entry Information
Gene Nameprobable steroid reductase DET2
Protein EntryDET2_ARATH
UniProt IDQ38944
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=2.5 uM for testosterone {ECO:0000269|PubMed:9108014}; KM=0.4 uM for progesterone {ECO:0000269|PubMed:9108014}; Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate {ECO:0000269|PubMed:9108014}; Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate {ECO:0000269|PubMed:9108014}; pH dependence: Optimum pH is 6.8. {ECO:0000269|PubMed:9108014};
Catalytic ActivityA 3-oxo-5-alpha-steroid + NADP(+) = a 3-oxo- Delta(4)-steroid + NADPH. {ECO:0000269|PubMed:10398719, ECO:0000269|PubMed:15993049, ECO:0000269|PubMed:9108014}.
Disruption PhenotypeBrassinolide-reversible dark- and light- grown defects; small dark-green dwarf plants with a limited leaves expension due to a reduced number of cells per leaf blade. {ECO:0000269|PubMed:10394910, ECO:0000269|PubMed:11867704, ECO:0000269|PubMed:8602526, ECO:0000269|PubMed:9401120}.
Enzyme RegulationInhibited by the 4-azasteroids 4-MA. {ECO:0000269|PubMed:9108014}.
FunctionInvolved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24- methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate. {ECO:0000269|PubMed:10398719, ECO:0000269|PubMed:11867704, ECO:0000269|PubMed:15993049, ECO:0000269|PubMed:8602526, ECO:0000269|PubMed:9108014, ECO:0000269|PubMed:9401120}.
PathwayPlant hormone biosynthesis; brassinosteroid biosynthesis.
SimilarityBelongs to the steroid 5-alpha reductase family. {ECO:0000305}.
Subcellular LocationMembrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.