LMPD Database

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LMP011420

UniProt Annotations

Entry Information
Gene NameType II inositol-1,4,5-trisphosphate 5-phosphatase FRA3
Protein EntryIP5P4_ARATH
UniProt IDQ84W55
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=63 uM for PtdIns(4,5)P2 (at pH 7.0 and 22 degrees Celsius); KM=299 uM for PtdIns(3,4,5)P3 (at pH 7.0 and 22 degrees Celsius); KM=1040 uM for PtdIns(1,4,5)P3 (at pH 6.5 and 22 degrees Celsius); Vmax=240 pmol/min/ug enzyme with PtdIns(4,5)P2 as substrate (at pH 7.0 and 22 degrees Celsius); Vmax=365 pmol/min/ug enzyme with PtdIns(3,4,5)P3 as substrate (at pH 7.0 and 22 degrees Celsius); Vmax=504 pmol/min/ug enzyme with PtdIns(1,4,5)P3 as substrate (at pH 6.5 and 22 degrees Celsius); pH dependence: Optimum pH is 6.5 with PtdIns(1,4,5)P3 as substrate, 7.5 with PtdIns(3,4,5)P3 as substrate, and 6.5-8.5 with PtdIns(4,5)P2 as substrate.; Temperature dependence: Optimum temperature is 22 degrees Celsius with PtdIns(1,4,5)P3 as substrate, and 22-37 degrees Celsius with PtdIns(4,5)P2 and PtdIns(3,4,5)P3 as substrates.;
Catalytic Activity1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate. {ECO:0000269|PubMed:15539468}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15539468};
FunctionRequired for secondary wall synthesis and actin organization in fiber cells. Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3. Has a higher substrate affinity toward PtdIns(4,5)P2. {ECO:0000269|PubMed:15539468}.
Sequence CautionSequence=AAB60921.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. {ECO:0000305}.
SimilarityContains 6 WD repeats. {ECO:0000305}.
Tissue SpecificityPredominantly expressed in interfascicular fibers and vascular bundles. Expressed in seedlings, stems, roots and flowers. Expressed at lower level in mature leaves. {ECO:0000269|PubMed:15539468}.