LMPD Database

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LMP012555

UniProt Annotations

Entry Information
Gene Namehepatocyte nuclear factor 4, alpha
Protein EntryHNF4A_RAT
UniProt IDP22449
SpeciesRat
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=Long; IsoId=P22449-1; Sequence=Displayed; Name=Short; IsoId=P22449-2; Sequence=VSP_003677;
FunctionTranscriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.
InteractionP02692:Fabp1; NbExp=3; IntAct=EBI-5261592, EBI-1209448;
MiscellaneousBinds fatty acids.
MiscellaneousDNA-binding activity of phosphorylated protein is reduced by fasting and by inducers of intracellular cyclic AMP.
PtmAcetylation at Lys-458 lowers transcriptional activation by about two-fold
PtmPhosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA (By similarity). Phosphorylated in the recognition sequence R-R-S-S near the DNA-binding domain; phosphorylation results in decrease in DNA-binding activity. Phosphorylation of HNF4 depends on the diet and is decreased by a carbohydrate-rich diet and is increased by fasting. {ECO:0000250, ECO:0000269|PubMed:9234678}.
PtmThe N-terminus is blocked.
Sequence CautionSequence=BAA01411.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ; Sequence=CAA40412.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ;
SimilarityBelongs to the nuclear hormone receptor family. NR2 subfamily
SimilarityContains 1 nuclear receptor DNA-binding domain
Subcellular LocationNucleus.
SubunitHomodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with PER2.
Tissue SpecificityLiver, kidney and intestine.