LMPD Database

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LMP012665

UniProt Annotations

Entry Information
Gene Namehematopoietic prostaglandin D synthase
Protein EntryHPGDS_RAT
UniProt IDO35543
SpeciesRat
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=100 uM for glutathione for the prostaglandin D synthase activity {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; KM=500 uM for glutathione for the glutathione-conjugating activity {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; KM=500 uM for PGH2 for the prostaglandin D synthase activity ; KM=3 mM for 1-chloro-2,4-dinitrobenzene ; Vmax=17.6 umol/min/mg enzyme with 1-bromo-2,4-dinitrobenzene as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Vmax=9.2 umol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Vmax=48.3 umol/min/mg enzyme with 1-fluoro-2,4-dinitrobenzene as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Vmax=17.9 umol/min/mg enzyme with 1-iodo-2,4-dinitrobenzene as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Vmax=0.35 umol/min/mg enzyme with cumene hydroperoxide as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Vmax=10.2 umol/min/mg enzyme with allyl isothiocyanate as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Vmax=11.3 umol/min/mg enzyme with benzyl isothiocyanate as substrate {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424};
Catalytic Activity(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15- hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy- 11-oxoprosta-5,13-dienoate.
Catalytic ActivityRX + glutathione = HX + R-S-glutathione.
CofactorName=glutathione; Xref=ChEBI:CHEBI:57925; Evidence={ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424}; Note=Glutathione is required for the prostaglandin D synthase activity. {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424};
FunctionBifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide. {ECO:0000269|PubMed:10871602, ECO:0000269|PubMed:11672424, ECO:0000269|PubMed:16547010, ECO:0000269|PubMed:9323136}.
Sequence CautionSequence=AAB72099.1; Type=Frameshift; Positions=73, 113; Evidence= ;
SimilarityBelongs to the GST superfamily. Sigma family
SimilarityContains 1 GST C-terminal domain
SimilarityContains 1 GST N-terminal domain
Subcellular LocationCytoplasm.
SubunitHomodimer
Tissue SpecificityHighly expressed in spleen and bone marrow. Lower levels of expression in small intestine, colon, liver, pancreas and skin. Not detected in brain, heart, lung or kidney (at protein level). {ECO:0000269|PubMed:11672424, ECO:0000269|PubMed:9323136}.