LMPD Database

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LMP012758

UniProt Annotations

Entry Information
Gene Namearachidonate 15-lipoxygenase
Protein EntryLOX15_RAT
UniProt IDQ02759
SpeciesRat
Comments
Comment typeDescription
Catalytic ActivityArachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate.
Catalytic ActivityArachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)- 15-hydroperoxyicosa-5,8,11,13-tetraenoate.
CofactorName=Fe cation; Xref=ChEBI:CHEBI:24875; Note=Binds 1 Fe cation per subunit.;
DomainThe PLAT domain can bind calcium ions; this promotes association with membranes
FunctionNon-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. {ECO:0000269|PubMed:15123652, ECO:0000269|PubMed:23382512, ECO:0000269|PubMed:8117750, ECO:0000269|PubMed:8444196}.
PathwayLipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
SimilarityBelongs to the lipoxygenase family
SimilarityContains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
SimilarityContains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Subcellular LocationCytoplasm, cytosol {ECO:0000269|PubMed:8117750}. Cell membrane ; Peripheral membrane protein . Lipid droplet . Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells (By similarity)
SubunitInteracts with PEBP1; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade
Tissue SpecificityDetected in leukocytes, lung and aorta