LMPD Database

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LMP012821

UniProt Annotations

Entry Information
Gene Namesynaptojanin 1
Protein EntrySYNJ1_RAT
UniProt IDQ62910
SpeciesRat
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=6; Comment=A stop codon is created in position 1309 of isoform 1 due to alternative splicing. ; Name=1; Synonyms=170 kDa; IsoId=Q62910-1; Sequence=Displayed; Name=2; Synonyms=145 kDa; IsoId=Q62910-2; Sequence=VSP_002686; Name=3; Synonyms=Delta-SAC; IsoId=Q62910-3; Sequence=VSP_002684; Name=4; Synonyms=170 kDa-16AA; IsoId=Q62910-4; Sequence=VSP_002685; Name=5; Synonyms=145 kDa-16AA; IsoId=Q62910-5; Sequence=VSP_002685, VSP_002686; Name=6; Synonyms=Delta-SAC-16AA; IsoId=Q62910-6; Sequence=VSP_002684, VSP_002685;
Catalytic Activity1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Developmental StageAt embryonic day 12 (E12) only isoform 1 is seen while at E16 and E18 isoform 1 and isoform 2 are seen. In the adult brain expression of isoform 2 increases dramatically as compared with its expression in embryonic brain where as isoform 1 decreases to undetectable levels.
DomainBinds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats
DomainSplicing of the SAC1 domain does not alter the catalytic activity of synaptojanin 1.
DomainThe C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
FunctionInositol 5-phosphatase which has a role in clathrin- mediated endocytosis.
InteractionQ12965:MYO1E (xeno); NbExp=2; IntAct=EBI-1149123, EBI-4279548; Q99962:SH3GL2 (xeno); NbExp=2; IntAct=EBI-1149123, EBI-77938; O35179:Sh3gl2; NbExp=2; IntAct=EBI-1149123, EBI-1149197; O35180:Sh3gl3; NbExp=2; IntAct=EBI-1149123, EBI-1149266;
SimilarityBelongs to the synaptojanin family
SimilarityContains 1 RRM (RNA recognition motif) domain
SimilarityContains 1 SAC domain. {ECO:0000255|PROSITE- ProRule:PRU00183}.
SimilarityIn the central section; belongs to the inositol 1,4,5- trisphosphate 5-phosphatase family
Subcellular LocationCytoplasm .
SubunitInteracts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). {ECO:0000250, ECO:0000269|PubMed:17257598, ECO:0000269|PubMed:9238017}.
Tissue SpecificityIsoform 1 is found in neonatal brain, and in a wide variety of adult non-neuronal tissues. Isoform 2 is expressed predominantly in the neurons, but is also found in all other tissues at much lower levels. Isoform 1 and isoform 2 are detected in the lung and heart. Isoform 1 is expressed at higher levels than isoform 2 in the testis and liver and both isoforms are not detected in the skeletal muscle. Isoform 3 with the 16-amino-acid insert is only found in the brain while isoform 3 without the 16- amino-acid insert is found in the lung.