LMPD Database

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LMP012863

UniProt Annotations

Entry Information
Gene Namephosphatidylinositol 4-kinase type 2 alpha
Protein EntryP4K2A_RAT
UniProt IDQ99M64
SpeciesRat
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=88 uM for ATP; KM=45 uM for PtdIns;
Catalytic ActivityATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
FunctionTogether with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. This lipid kinase is the major phosphatidylinositol 4-phosphate (PI4P) producer in the Golgi apparatus, it generates more than 50% of this molecule which is essential for the identity of the organelle, protein sorting and membrane trafficking
PtmPalmitoylated by ZDHHC3 and ZDHHC7 in the CCPCC motif. Palmitoylation is cholesterol-dependent, and required for TGN localization (By similarity)
SimilarityBelongs to the PI3/PI4-kinase family. Type II PI4K subfamily
SimilarityContains 1 PI3K/PI4K domain
Subcellular LocationCytoplasm . Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor {ECO:0000250}. Membrane raft . Cell projection, dendrite . Cell junction, synapse, presynaptic cell membrane . Cell junction, synapse, synaptosome {ECO:0000250}. Mitochondrion . Endosome . Cytoplasmic vesicle . Note=Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity). Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner
SubunitAssociates with the BLOC-1 and the AP-3 complexes; the BLOC-1 complex is required for optimal binding of PI4K2A to the AP-3 complex. Interacts with BLOC1S5 and DTNBP1 (By similarity). Interacts with FOS; this interaction may enhance phosphatidylinositol phosphorylation activity (By similarity)