| Comment type | Description |
|---|
| Biophysicochemical Properties | Kinetic parameters: KM=6.0 uM for PtdIns(4,5)P2 ; Vmax=10 umol/min/mg enzyme ; |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ; |
| Domain | The Ras-associating domain 1 is degenerated and may not bind HRAS (By similarity). The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A and probably RAP2A and RAP2B and recruitment of HRAS to the cell membrane |
| Domain | The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway (By similarity) |
| Enzyme Regulation | Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. {ECO:0000269|PubMed:11179219, ECO:0000269|PubMed:11641393, ECO:0000269|PubMed:11788587, ECO:0000269|PubMed:12900402, ECO:0000269|PubMed:15322077}. |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine- exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. {ECO:0000269|PubMed:12721365, ECO:0000269|PubMed:16314422, ECO:0000269|PubMed:17086182}. |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}. |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}. |
| Similarity | Contains 1 Ras-GEF domain. {ECO:0000255|PROSITE- ProRule:PRU00168}. |
| Similarity | Contains 2 Ras-associating domains |
| Subcellular Location | Cytoplasm, cytosol . Cell membrane {ECO:0000250}. Golgi apparatus membrane . Note=Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Associates with Golgi membranes (By similarity) |
| Subunit | Interacts with GTP-bound HRAS, RAP1A and probably with RAP2A, RAP2B and RRAS. Interacts with RHOA. Interacts with IQGAP1 (By similarity) |
| Tissue Specificity | Ubiquitously expressed. Detected in glomerular podocytes (at protein level). {ECO:0000269|PubMed:11179219, ECO:0000269|PubMed:17086182}. |