LMPD Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
LMP013003

UniProt Annotations

Entry Information
Gene Namehydroxysteroid (17-beta) dehydrogenase 6
Protein EntryH17B6_RAT
UniProt IDO54753
SpeciesRat
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.1 uM for 5-alpha-androstan-3-alpha,17-beta-diol ; KM=0.2 uM for androsterone ; KM=0.5 uM for dihydrotestosterone ; KM=1.1 uM for testosterone ; KM=0.8 uM for estradiol ; KM=3 uM for NAD ; KM=1 mM for NADP ; Vmax=2.5 nmol/min/mg enzyme for 5-alpha-androstan-3-alpha,17- beta-diol ; Vmax=0.1 nmol/min/mg enzyme for androsterone ; Vmax=0.5 nmol/min/mg enzyme for dihydrotestosterone ; Vmax=1.1 nmol/min/mg enzyme for testosterone ; Vmax=2.1 nmol/min/mg enzyme for estradiol ; Note=Vmax was measured using transfected cell lysates.;
Catalytic Activity17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H.
Catalytic ActivityAll-trans-retinol-[cellular-retinol-binding- protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding- protein] + NADH.
Catalytic ActivityTestosterone + NAD(+) = androstenedione + NADH.
Enzyme RegulationCompetitively inhibited by 9-cis-retinoic ancid and 13-cis-retinoic acid
FunctionNAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has retinol dehydrogenase activity towards all-trans- retinol (in vitro) (By similarity). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro)
InductionUp-regulated by testosterone. Levels are very low in castrated male rats
Sequence CautionSequence=AAB88253.1; Type=Erroneous initiation; Evidence= ; Sequence=AAH88104.1; Type=Erroneous initiation; Evidence= ; Sequence=AAH91114.1; Type=Erroneous initiation; Evidence= ;
SimilarityBelongs to the short-chain dehydrogenases/reductases (SDR) family
Subcellular LocationMicrosome membrane ; Peripheral membrane protein {ECO:0000250}; Lumenal side . Endoplasmic reticulum membrane ; Peripheral membrane protein {ECO:0000250}; Lumenal side .
Tissue SpecificityDetected in prostate, liver and kidney