Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Introduction | Browse | Download
Mouse-over the above links for more information

Details:

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
PA| PC| PE| PG| PI| PS| DNA| TNF

Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(32:0) -
94.6
-
48.2		 76
82.1
59.8
56.8		 75.3
59.1
64.8
66.1		 47.2
47.5
51.4
70.2		 56.7
51.3
52
41.3		 81.2
65.5
80.9
51.2		 121
95.5
75.3
56		 118
73.4
132
55		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(32:0) -
73.4
-
134		 60.8
108
127
60.8		 78.7
114
62.3
117		 80.3
81.1
130
65.4		 85.3
87.3
131
95.5		 95.4
97
155
91.1		 102
86.7
147
84.3		 177
107
125
95.9		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(32:0) -
43.8
-
48.7		 34.9
46.2
44.5
39.7		 -
42
41.3
48.9		 21.4
-
24.7
22		 31.6
27.2
-
38.3		 50.2
66.5
38
14.3		 60.4
58.7
110
84.2		 68.8
46.3
88
53		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(32:1) -
83.4
-
29.5		 46.7
47
38.1
40.2		 45.5
40.5
50.2
49.4		 29.2
28.4
33.3
46.7		 28.3
29.7
27.9
25.2		 33.5
33
33
29.1		 51.5
58.1
31.6
34.5		 48
52.2
54.1
35.8		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(32:1) -
36.5
-
99.9		 29.7
50
57.1
30		 37
53
29.3
54.5		 39
37.5
64.7
35.2		 40.1
42.5
63
44.8		 42.4
49.4
67.6
44.1		 37.5
48
52.3
37.6		 92.8
67.6
49.5
51.9		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(32:1) -
23.8
-
45.7		 26.9
35.8
33.1
28.1		 -
49.4
45.3
36.8		 9.05
-
14.7
15.2		 24.2
9.81
-
30.6		 28.8
32.3
7.86
4.08		 28.5
31.9
33.7
52.6		 16.3
15.7
21.2
23.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(34:1) -
171
-
109		 169
203
143
140		 155
136
177
166		 85.5
58.3
92.7
190		 61.8
62.4
62.4
51.2		 79.9
74.4
73.8
63.6		 191
222
68.1
70.5		 200
184
176
167		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(34:1) -
97
-
170		 89.7
148
190
75.2		 118
163
82.3
172		 119
120
226
102		 119
124
165
146		 142
124
119
104		 88.4
91.1
152
109		 194
127
87.1
105		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(34:1) -
114
-
114		 96
123
119
115		 -
59.7
90.4
82.8		 33.8
-
41.3
31		 34.6
38.3
-
49.5		 36.3
96.5
33.9
14.9		 96.1
105
112
116		 107
50.5
98.2
94.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(34:2) -
41.4
-
18.9		 29.3
27.4
21.7
24.3		 34.8
19.6
26.3
33.2		 16.4
15.1
17.1
25		 15.2
15.8
15.9
12.9		 15.3
15.1
13.7
13.6		 23.8
36.4
13.5
16.7		 30.8
25.9
25.4
22.6		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(34:2) -
15.2
-
30.5		 16.5
23.1
25.2
14.7		 19
24.2
14.6
26.8		 19.4
19.8
32.4
18.6		 16.6
18.9
24.5
21.6		 20.7
17.3
17.1
15.1		 13.1
15.2
21.7
15.9		 24.3
22
13.5
13.2		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(34:2) -
9.21
-
12.5		 11
11.5
13.4
14.1		 -
7.9
6.18
2.92		 -
-
2.85
1.48		 0.68
-
-
4.53		 1.42
7.08
-
-		 1.96
3.57
5.43
8.71		 -
-
0.28
1.97		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(36:0) -
40.8
-
62.5		 38.6
71.6
90.3
33.4		 43.8
71
39.8
75.1		 49.6
46.2
87
41		 41.7
49.1
64
56.1		 25.5
19.6
11.6
10.2		 14.1
16.4
24.2
14.6		 26.6
19.6
11.1
12.8		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(36:0) -
45.9
-
27.5		 24.4
35.7
21.4
32.5		 -
-
-
-		 -
-
10.6
-		 8.69
-
-
12.2		 2.9
-
-
-		 -
-
-
-		 -
-
-
7.14		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(36:1) -
247
-
184		 320
327
249
233		 306
244
334
317		 136
65.9
146
370		 75.5
77.6
81.9
59		 85.9
84.9
74.4
71.7		 335
443
77
93		 330
308
326
290		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(36:1) -
190
-
360		 156
281
357
128		 187
262
139
297		 192
196
366
169		 199
197
255
235		 258
223
173
151		 152
151
259
184		 383
251
132
178		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(36:1) -
187
-
226		 174
213
197
184		 -
73.1
118
99.9		 45.4
-
54.4
30.1		 50.6
52.8
-
56.1		 49.5
134
41.5
20.5		 137
125
131
118		 108
63.1
165
137		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(36:2) -
114
-
78.1		 137
143
109
104		 135
119
136
137		 69.4
36.8
69.5
156		 39.8
41.2
42.5
33.8		 43
41.4
37.3
37.5		 134
174
35.2
42.5		 115
133
127
122		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(36:2) -
91.2
-
173		 86.3
132
150
68.1		 94.7
125
74.7
137		 96.7
101
160
91.5		 93.9
94.8
123
115		 117
107
86.6
80.3		 73.5
79.2
109
88.7		 132
101
65.5
80.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(36:2) -
87.2
-
101		 83.7
98.5
102
93.1		 -
32.2
65.4
42.4		 16.4
-
22.8
9.37		 20.4
19.4
-
26.7		 15.9
57.4
14.7
-		 46.4
35.6
48.6
42.9		 28.7
16.6
62.6
60.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(38:3) -
47
-
32.2		 49.1
47.7
34.8
34.2		 34
30.2
43.7
45.4		 19
12.6
20.3
42.1		 14.5
13.8
13.8
12.1		 16.4
14.5
15
13.2		 49.8
61
16.1
16.1		 51
38.9
49
47.8		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		 PA(38:4) -
42.2
-
33.5		 52.5
52.4
39.3
41		 37.9
35.5
50.5
46		 19.9
10.4
21
45.9		 11.3
12.7
13.3
9.87		 11.9
11.8
10.5
11.1		 44.3
49
12.5
13.2		 41.5
45.1
46.9
52.9		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		 PA(38:4) -
44.2
-
91.4		 35.2
56.4
75.5
37.8		 37.5
53.8
32.4
61.4		 36.3
41.1
70.9
35.4		 34.7
38.1
56.6
54.7		 39.9
38.2
53.5
76.5		 24.1
23.1
50.2
32.4		 55.4
52.1
30
31.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		 PA(38:4) -
27
-
34.3		 19.3
20.7
27.8
25.5		 -
-
7.02
-		 -
-
-
-		 -
-
-
-		 -
0.07
-
-		 8.93
1.74
8.87
10.6		 6.63
-
7.54
7.14		 pmol/sample