Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 PA| PC| PE| PG| PI| PS| DNA| TNF Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(32:1) - 15.6 - 18.2 8.36 11.3 7.75 5.6 11.4 10.2 8.74 9.25 13.1 26.3 12.1 9.18 23.3 26.3 20.8 20.2 34.1 30 31 27.2 12.7 16.3 30.4 28.7 22.9 8.64 13.4 10.6 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(32:1) - 23.8 - 31.2 25.2 34.4 37.1 22.7 31 37.4 21.3 31.1 35.8 35.6 40.1 28.3 31.9 37.9 36.1 31.5 23.1 16.6 19.2 26.8 21.9 23 26.7 18 34.6 22.6 33.1 25.7 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(32:1) - 15.4 - 11.4 12.6 16.5 12.6 12.4 - 7.15 5.72 9.35 17.5 - 12.8 16.5 13.9 16.5 - 19.6 14.9 13.5 14.1 5.54 6.76 5.73 9.47 8.36 9.89 7.07 12.9 16.5 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(34:1) - 114 - 212 78.8 116 92.7 90.3 92.1 84.8 108 88.4 114 170 137 108 164 168 166 151 225 207 211 188 144 195 207 206 165 123 189 127 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(34:1) - 231 - 262 217 327 362 213 294 335 212 330 298 280 409 242 290 309 322 301 196 160 204 233 200 177 226 153 296 169 258 193 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(34:1) - 145 - 95.1 113 142 114 121 - 51.5 45.2 82.4 129 - 98.9 150 94.5 126 - 120 105 142 106 63.2 81 56.4 116 84.4 120 58.3 129 159 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(34:2) - 44 - 61.6 29.4 31.5 29.8 27.2 33.9 24.6 36.1 30 40.3 41.7 41.8 42.9 39.5 42.3 38.8 36.9 48.8 48.6 44.1 41.5 46.9 87.2 42.9 49.1 64.7 54.4 60 56.8 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(34:2) - 107 - 98 98.6 137 149 92 114 128 89.9 120 120 114 149 102 121 126 119 112 84.6 77.5 75.5 89.2 86.5 96.7 78.8 64.9 123 89.5 102 90.6 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(34:2) - 64.7 - 46.9 68.9 91.8 65.9 64.1 - 32.6 35.3 48.3 70.1 - 43.3 73.8 46.6 61.9 - 66.4 53.7 70.8 44.7 30.8 32.2 28 49.9 52.7 41.6 34.6 62.7 100 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(36:1) - 98.1 - 243 59.6 78.4 63.2 52.6 58 55.2 56.1 63.5 104 160 159 80.6 172 179 154 142 196 192 183 164 117 125 171 184 142 119 128 103 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(36:1) - 434 - 369 415 653 720 332 558 692 444 647 606 513 681 471 482 531 551 540 172 144 175 370 192 207 183 131 285 189 255 211 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(36:1) - 229 - 154 123 173 138 179 - 43.6 48.8 88.5 220 - 275 185 277 216 - 344 270 270 251 55.5 68.4 48.1 85.4 61.1 120 63 112 295 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(36:2) - 613 - 708 350 399 383 393 412 336 419 379 455 349 502 564 371 387 367 322 391 390 375 365 537 884 337 402 688 656 724 617 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(36:2) - 1576 - 1328 1356 1934 2207 1287 1603 1834 1337 1919 1610 1467 2102 1507 1583 1611 1658 1622 939 852 839 1259 992 1088 877 727 1227 981 1040 980 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(36:2) - 815 - 672 844 1083 884 853 - 418 462 639 798 - 580 981 617 829 - 777 707 1145 604 507 525 490 694 669 678 500 734 1408 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(36:3) - 106 - 144 62.7 61.5 82.3 83.2 54 60.3 92.2 72.3 81.9 57.8 103 124 51.8 58.1 69.5 58.6 58.4 59.9 67.7 66.9 63.5 115 54.8 66.6 59.6 81.6 98.5 86.7 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(36:3) - 226 - 229 216 274 401 218 247 282 249 345 231 236 365 255 223 236 288 265 140 142 159 205 127 161 137 129 141 145 141 161 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(36:3) - 127 - 123 158 193 174 167 - 65.2 90.6 130 130 - 105 188 89.5 127 - 136 92.1 143 89.9 80.8 61.2 53.1 88.3 94.7 69.1 52.1 96.1 179 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(38:1) - 6.65 - 6.34 2.15 4.78 3.15 3.2 0.86 2.6 3.73 3.34 4.9 16.3 7.47 2.82 13.2 12.6 8.76 15 18.1 18.2 19.9 19.4 2.57 5.97 21.1 16.5 5.42 4.62 5.89 4.04 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(38:1) - 18.8 - 37 16.6 29.3 29.5 15.6 21.1 28.8 19.3 29.5 25.4 22.2 23.6 17.1 15.9 19.6 17.4 24.3 8.04 2.88 3.46 11.2 6.92 10.8 7.29 4.16 14.7 7.51 13.2 8.74 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(38:1) - 6.49 - 7.61 5.01 6.53 4.26 5.9 - 1.49 1.56 3.51 10.1 - 18.4 6.09 17 7.97 - 20.8 14.3 14.3 12 1.51 2.32 2.34 2.37 2.13 5.72 2.78 4.54 8.84 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(38:2) - 67.2 - 70 26.4 37.2 31.6 35 33.9 27.7 30.6 27.7 53 79.1 67.1 53.5 82.1 76.3 76.8 78.2 85.8 89.6 91.9 86.9 37.8 54.4 83 92.6 61.1 55.9 52.7 48.5 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(38:2) - 239 - 195 277 425 453 199 333 402 309 395 338 286 409 302 258 304 314 313 111 89.3 102 206 114 133 115 81.1 150 110 141 113 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(38:2) - 120 - 90.9 80.9 113 92.2 102 - 32.3 37.3 59.5 99.5 - 115 107 122 95.2 - 155 122 140 111 37.9 40.5 31.8 63.6 49.9 53.4 38.5 69.5 157 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(38:3) - 57.2 - 103 47.9 62.9 67.4 54.4 56.2 39.7 62.8 63.3 80.2 113 123 81.3 114 110 120 110 110 118 125 118 64.4 97.2 109 123 57.1 67.5 85.2 84.9 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(38:3) - 372 - 356 409 624 757 325 481 592 479 652 456 411 666 479 382 436 536 516 153 135 153 336 164 189 158 133 171 155 168 175 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(38:3) - 172 - 145 128 170 164 170 - 48.8 67.7 106 135 - 167 181 160 148 - 225 158 188 159 65.1 54 50.6 84.2 73.3 65.9 59.6 86.8 235 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(38:4) - 74.1 - 82.8 41.9 49.1 60.8 60.1 30.1 43.4 56.9 50.5 65.5 93.9 117 67.6 83.5 92.2 99 89.2 82.2 88.4 103 97.2 40.5 70 84.3 96 28.8 38.9 42.2 53 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(38:4) - 286 - 275 309 443 626 287 356 451 403 513 333 309 556 410 278 330 421 441 107 101 127 262 97.1 135 124 98.8 94.4 85.7 120 113 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(38:4) - 144 - 121 106 142 146 159 - 47.4 57.3 96.7 118 - 146 161 119 124 - 192 119 149 130 55.3 38.1 40.2 65 59.5 43.3 37.2 65.7 171 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(38:5) - 45.8 - 47.6 26.3 37.8 44.4 38.7 36.8 20.3 37.9 43.7 41.6 64.3 85.1 51.5 54 66.6 67.4 69.7 59.6 62.7 72.8 66.1 26.3 40.6 60.7 63.5 9.66 21.2 49.8 38.7 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(38:5) - 180 - 176 196 282 399 182 224 266 244 334 223 201 345 251 170 198 276 256 68.6 64.7 83.5 177 56.5 65.2 82.9 65.3 55.3 50 77 77.7 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(38:5) - 92.5 - 91.1 76.1 102 103 117 - 31.1 44.1 66.7 81.7 - 99.9 109 77.1 83.8 - 127 75 89.7 84.4 35.8 25.8 20.8 43.8 42.8 27.5 21.7 42.5 99.7 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(40:3) - 9.2 - 16.2 3.28 4.16 7.53 5.07 4.29 4.02 3.41 5.57 8.62 15.3 11.4 9.79 12.4 15.3 11.8 18.3 15.6 17.7 21.1 21.6 3.75 3.4 18.2 18.6 3.65 4.11 3.05 5.95 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(40:3) - 48.4 - 64.9 52.1 84.6 105 46.5 65.1 79.1 65.8 89.9 62.7 49.8 77 60.5 48.9 60.7 67.4 76.1 14.2 13.3 21 39.4 17.9 18.1 20.8 11.4 23.4 14.8 22.9 20.8 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(40:3) - 20 - 19.2 12.2 17.2 15 20.2 - 6.53 7.22 10 16.8 - 28.8 14.5 27.3 13.7 - 31.1 19.9 30.4 19.7 4.98 6.25 4.9 8.93 6.38 5.4 5.61 8.52 22.9 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(40:4) - 22.1 - 30.7 4.74 10.2 9.55 12 9.81 4.73 11.4 9.47 11.2 25.2 21.1 8.08 22.3 21.6 19.9 28.5 21.1 22.9 32.1 28.2 1.88 12.7 27.8 25.8 4.71 3.68 16.4 11 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(40:4) - 62.5 - 98.7 72.9 107 147 64.8 83 103 101 141 76.4 82.9 130 97.6 62.2 63.2 104 94.2 19.1 15.5 23.4 56.1 19.3 24.6 21.1 21.1 17.5 20.2 27.6 27.6 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(40:4) - 27.6 - 30.6 19.8 24 25.1 30.5 - 7.53 8.39 16.1 24.6 - 50.5 27 33.5 23.9 - 46.9 27.5 42.1 30.5 9.29 6.4 7.05 12.6 11.5 9.19 7.27 14.8 33.3 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(40:5) - 30.1 - 50.1 12.2 22 22.6 19.4 18.3 12 14.6 23 21.1 39.1 39.7 26.1 37.5 37.9 45.6 51 33 36.4 59.6 58.4 14.1 25.9 49.7 50 7.54 13.4 36.6 25.4 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(40:5) - 131 - 161 119 201 304 131 151 191 189 262 139 137 257 195 106 129 201 203 44.4 38.4 51.5 111 41.6 46 51.6 41.5 38.5 30.8 58.5 45.6 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(40:5) - 57.4 - 71.3 44.5 58.5 58.8 59 - 19.5 24.4 38.4 45.4 - 106 68.7 70.9 49.7 - 120 51.1 77.2 73.6 24.3 15.6 14.8 26.8 20.8 14 15.9 30.9 78.8 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(40:6) - 43.2 - 83.2 29.4 36.5 43.8 42.1 16.3 19.6 48.4 37.4 39.2 69.2 79.3 60 58.6 68.3 82.9 88.6 61.7 68.8 109 103 18.9 36.1 88.2 91.5 15.8 24.7 63.4 70.4 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(40:6) - 195 - 292 218 361 564 219 247 325 360 442 246 230 476 332 173 215 353 353 62.8 70.6 98.9 204 68.9 80.4 103 73.5 67.3 58.7 112 109 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(40:6) - 130 - 152 98.6 136 140 153 - 36.8 53.1 89 110 - 248 141 155 111 - 286 120 169 173 52.1 38.6 29.9 74.7 72.4 51.5 37.2 86.3 193 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(40:7) - 70 - 99.6 35.2 49.1 74.5 73.9 36.6 43.3 59.1 69.2 56.9 93.4 119 71.9 77.1 84.6 110 115 75.1 86.8 125 123 37.9 61 107 122 39.9 37.3 84.5 89.8 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(40:7) - 247 - 344 278 430 729 309 315 421 435 595 323 290 582 405 243 275 448 440 88.8 86.9 136 274 82.2 98.7 146 110 86.8 74.3 151 144 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(40:7) - 213 - 263 165 219 243 260 - 67.4 106 163 177 - 333 264 221 178 - 411 191 264 288 108 63.7 60.8 147 132 69.6 65.4 153 333 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) PG(42:10) - 9.98 - 15.7 5.65 10.9 7.97 7.4 6.87 7.92 10.9 8.25 15.9 24.9 15.2 9.18 25.9 25.4 25.6 24.5 22.4 20.2 23.1 25.1 5.14 13.6 20.9 24 3.06 8.21 3.76 5.05 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) PG(42:10) - 48.2 - 66.1 46.3 73.9 94.3 45.6 53.5 66.2 59 85.9 48.2 39.5 68.5 52.2 41.8 40.1 50 51.6 15.5 16 20.8 28.8 19.5 19.7 16.7 14.1 25.9 17.9 17.6 17.5 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) PG(42:10) - 33.2 - 28.9 30.1 33.6 35.8 33.4 - 13.7 17.2 23.2 55.6 - 76 51.4 71.3 59.4 - 97.5 56.3 66.1 68.6 15.2 9.71 12.6 20.3 15.9 16.9 13.8 17.2 50.9 pmol/sample