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Peritoneal macrophages were obtained from thioglycolate-stimulated mice, as per protocols PP0000001401, PP0000001502 and PP0000001801 (pdf). Cells were treated with Kd02-Lipid A and/or compacin; measurements were taken at 30min, 1h, 2h, 4h, 8h, 12h, and 24h.
Data normalization was performed as described in J Mol Endocrinol Sasik et al. 33 (1): 1 (PDF)
45,214 spots on the array