Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin

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Details/Graph
LM_ID NameMass
(neutral)		 Kdo+Comp
Kdo
Comp
Ctrl
 0 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 0.5 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 1 hr		 Kdo+Comp
Kdo
Comp
Ctrl
 2 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 4 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 8 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 12 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 24 hrs
Units
. . . .
  LMFA01030283 10Z-heptadecenoic acid268.2 (ions) -
-
-
0.57		 0.73
0.51
0.6
0.683		 0.977
0.953
0.587
0.65		 0.53
0.597
0.61
0.64		 0.967
0.74
0.68
0.577		 0.73
0.673
0.74
0.45		 0.613
0.667
0.5
0.61		 0.767
0.693
0.697
0.363		 pmol/ug DNA
  LMFA01030159 11,14,17-eicosatrienoic acid306.3 (ions) -
-
-
4.87		 5.29
5.19
4.16
4.56		 5.17
6.27
3.46
3.95		 4.53
4.72
3.65
4.02		 4.88
4.83
3.61
3.71		 3.87
3.8
4.22
3.62		 3.59
3.62
4.65
3.89		 3.71
3.16
7.83
6.68		 pmol/ug DNA
  LMFA01030130 11,14-eicosadienoic acid308.3 (ions) -
-
-
0.257		 0.303
0.34
0.28
0.233		 0.207
0.27
0.22
0.247		 0.287
0.283
0.203
0.233		 0.227
0.253
0.237
0.217		 0.237
0.173
0.29
0.247		 0.303
0.263
0.263
0.183		 0.387
0.253
0.23
0.207		 pmol/ug DNA
  LMFA01030408 13,16,19-docosatrienoic acid334.3 (ions) -
-
-
-		 0.017
0.01
0.02
0.02		 0.01
0.01
0.02
0.03		 0.01
0.04
-
0.025		 0.01
0.015
0.013
0.01		 0.02
0.02
0.01
0.01		 0.017
0.01
0.01
0.015		 0.03
0.017
0.02
0.017		 pmol/ug DNA
  LMFA01030132 13,16-docosadienoic acid336.3 (ions) -
-
-
0.02		 0.015
0.02
0.02
0.01		 0.025
0.03
0.015
0.02		 0.01
0.01
0.023
0.02		 0.027
0.02
0.04
0.01		 0.015
0.025
0.027
0.015		 0.015
0.025
0.02
0.025		 0.017
0.017
0.035
0.015		 pmol/ug DNA
  LMFA01030759 5,8,11,14,17-eicosapentaenoic acid302.2 (ions) -
-
-
1.02		 0.97
0.947
0.98
0.99		 0.903
0.973
0.77
0.847		 0.883
0.793
0.74
0.823		 0.6
0.817
0.703
0.69		 0.557
0.553
0.727
0.647		 0.477
0.487
0.7
0.59		 0.493
0.463
0.8
0.653		 pmol/ug DNA
  LMFA01030157 5,8,11-eicosatrienoic acid306.3 (ions) -
-
-
0.017		 0.02
0.02
0.02
0.02		 0.03
0.017
0.033
0.017		 0.02
0.02
0.017
0.027		 0.027
0.013
0.03
0.017		 0.023
0.02
0.02
0.015		 0.03
0.033
0.03
0.02		 0.047
0.037
0.01
0.013		 pmol/ug DNA
  LMFA01030184 7,10,13,16,19-docosapentaenoic acid330.3 (ions) -
-
-
3.1		 2.78
2.78
2.83
2.86		 2.42
2.6
2.12
2.31		 2.44
2.32
2.16
2.22		 2.22
2.26
2
2.12		 1.52
1.47
1.98
1.81		 1.59
1.58
2.01
1.5		 1.8
1.49
2.4
1.89		 pmol/ug DNA
  LMFA01030178 7Z,10Z,13Z,16Z-docosatetraenoic acid332.3 (ions) -
-
-
1.32		 1.31
1.33
1.22
1.25		 1.07
1.31
0.903
1.03		 1.12
1.07
1.06
1.08		 1.42
1.2
0.927
0.97		 0.857
0.737
0.92
0.81		 0.733
0.64
0.95
0.737		 0.703
0.61
1.38
0.873		 pmol/ug DNA
  LMFA01030056 9Z-palmitoleic acid254.2 (ions) -
-
-
8.01		 10.2
7.83
8.07
7.02		 12.7
10.3
7.87
7.97		 7.21
8.96
8.49
7.89		 9.85
7.68
8.55
8.32		 7.43
7.16
9.94
7.18		 7.32
8.28
7.3
9.04		 9.26
8
7.38
4.87		 pmol/ug DNA
  LMFA01030152 alpha-linolenic acid278.2 (ions) -
-
-
0.377		 0.297
0.33
0.37
0.343		 0.373
0.367
0.287
0.317		 0.303
0.3
0.257
0.313		 0.31
0.31
0.253
0.283		 0.32
0.313
0.307
0.207		 0.27
0.27
0.227
0.22		 0.267
0.25
0.21
0.12		 pmol/ug DNA
  LMFA01010020 Arachidic acid312.3 (ions) -
-
-
0.787		 1.43
1.32
1.38
1.29		 0.67
0.887
1.04
1.1		 1.06
1.21
0.917
1.23		 1.17
1.62
1.43
1.24		 1.17
1.08
1.28
1.14		 1.29
1.42
1.25
0.847		 1.48
1.68
1.2
0.71		 pmol/ug DNA
  LMFA01030001 Arachidonic acid304.2 (ions) -
-
-
9.32		 13.3
12.9
11.8
9.95		 12.7
14.7
9.35
10.6		 11.7
11.4
9.32
10.5		 9.61
10.4
9.03
9.36		 5.65
5.56
9.27
8.29		 4.88
5.06
9.43
7.19		 4.53
4.18
11.2
8.83		 pmol/ug DNA
  LMFA01010022 Behenic acid340.3 (ions) -
-
-
0.317		 0.447
0.573
0.483
0.443		 0.537
0.63
0.433
0.46		 0.457
0.453
0.463
0.7		 0.52
0.473
0.513
0.41		 0.35
0.373
0.567
0.393		 0.38
0.407
0.44
0.353		 0.41
0.483
0.407
0.31		 pmol/ug DNA
  LMFA01030158 bishomo-gamma-linolenic acid306.3 (ions) -
-
-
1.32		 1.33
1.19
1.2
1.19		 1.14
1.24
1.01
1.06		 1.06
1.03
0.99
1.12		 1.22
1.04
0.953
0.983		 0.803
0.733
1.08
0.887		 1.01
0.837
1.08
0.897		 1.09
0.783
1.22
0.88		 pmol/ug DNA
  LMFA01010026 Cerotic acid396.4 (ions) -
-
-
0.37		 0.227
0.577
0.373
0.283		 0.335
0.28
0.465
0.33		 0.42
0.36
0.245
0.48		 0.615
0.26
0.575
0.457		 0.565
0.415
0.395
0.56		 0.773
0.713
0.48
0.235		 0.797
0.883
0.29
0.247		 pmol/ug DNA
  LMFA01030089 cis-erucic acid338.3 (ions) -
-
-
0.03		 0.03
0.02
0.027
0.037		 0.033
0.05
0.033
0.027		 0.02
0.033
0.03
0.057		 0.03
0.04
0.037
0.027		 0.04
0.033
0.04
0.023		 0.027
0.043
0.037
0.06		 0.06
0.047
0.097
0.06		 pmol/ug DNA
  LMFA01030092 cis-selacholeic acid366.3 (ions) -
-
-
0.107		 0.087
0.08
0.087
0.097		 0.143
0.127
0.09
0.093		 0.07
0.083
0.113
0.12		 0.113
0.1
0.113
0.077		 0.11
0.12
0.153
0.07		 0.073
0.11
0.08
0.12		 0.107
0.097
0.15
0.113		 pmol/ug DNA
  LMFA01030185 DHA328.2 (ions) -
-
-
4.83		 3.99
3.92
4.15
4.33		 3.6
3.72
3.19
3.59		 3.45
3.28
3.19
3.42		 3.39
3.24
2.95
2.99		 2.25
2.34
2.74
2.46		 2.29
2.45
2.75
2.13		 2.69
2.34
2.98
2.33		 pmol/ug DNA
  LMFA01030141 gamma-linolenic acid278.2 (ions) -
-
-
0.26		 0.157
0.17
0.207
0.193		 0.163
0.28
0.18
0.163		 0.243
0.163
0.153
0.193		 0.23
0.253
0.203
0.157		 0.153
0.157
0.19
0.105		 0.24
0.17
0.137
0.137		 0.193
0.167
0.087
0.11		 pmol/ug DNA
  LMFA01010012 Lauric acid200.2 (ions) -
-
-
3.8		 3.76
4.21
3.94
5.12		 3.06
5.86
4.41
3.53		 5.26
3.72
3.64
5.59		 3.88
3.81
4.35
4.25		 3.32
4.43
3.6
4.09		 5.43
3.85
4.04
3.48		 4.56
5.62
2.86
2.81		 pmol/ug DNA
  LMFA01010024 Lignoceric acid368.4 (ions) -
-
-
0.71		 1.32
1.53
1.43
1.44		 1.2
1.03
0.797
0.97		 1.2
1.3
0.943
1.16		 1.13
1.31
1.63
1.47		 1.45
1.26
1.67
1.53		 1.99
1.84
1.32
0.977		 2.11
2.33
1.66
1.5		 pmol/ug DNA
  LMFA01030120 Linoleic acid280.2 (ions) -
-
-
7.51		 7.12
6.25
6.91
7.1		 5.96
7.88
5.4
5.41		 7.12
5.46
5.4
5.85		 6.85
6.37
5.99
5.48		 5.85
5.77
5.99
4.95		 7.18
5.93
5.93
4.67		 7.61
5.06
4.73
3.92		 pmol/ug DNA
  LMFA01010017 Margaric acid270.3 (ions) -
-
-
2.93		 2.61
3.12
2.86
3.27		 2.01
3.44
2.52
2.75		 2.58
2.37
2.54
2.79		 2.95
3.14
2.09
2.82		 2.46
2.51
2.7
2.23		 2.7
2.69
2.45
1.7		 2.97
3.1
2.46
1.57		 pmol/ug DNA
  LMFA01010014 Myristic acid228.2 (ions) -
-
-
15.9		 13.4
14
14.7
14.8		 13
20.4
11.2
13.3		 16.3
13.4
13.5
16.6		 14.7
13.3
14.5
15.9		 12.9
16.4
13.2
14.4		 16.9
16.6
13.4
11.9		 24.6
25.5
16.8
12.5		 pmol/ug DNA
  LMFA01030002 Oleic acid282.3 (ions) -
-
-
194		 198
179
180
190		 174
194
142
163		 168
159
152
151		 174
171
173
162		 170
162
181
151		 185
178
191
160		 229
182
231
159		 pmol/ug DNA
  LMFA01010001 Palmitic acid256.2 (ions) -
-
-
114		 115
114
118
90.5		 88.5
138
82.4
100		 117
105
98.7
105		 131
119
112
104		 135
120
108
109		 167
162
115
93.1		 206
173
122
79.2		 pmol/ug DNA
  LMFA01010015 Pentadecanoic acid242.2 (ions) -
-
-
2.52		 2.82
3.35
3.74
3.82		 2.73
4.22
2.43
3.11		 3.97
3.14
2.94
4.25		 3.92
2.97
3.43
2.86		 2.46
2.98
3.21
3.74		 2.87
2.39
2.63
2.1		 2.97
4.21
3.19
1.92		 pmol/ug DNA
  LMFA01010018 Stearic acid284.3 (ions) -
-
-
73		 64.8
69.6
65.9
64.1		 56.2
85.5
56.9
64.5		 63.7
59.6
57.7
76.3		 69.4
64.1
55.7
58.1		 70.6
73
63.3
59.3		 91
87
66.1
55.7		 117
95.6
89.1
61		 pmol/ug DNA
  LMFA01030357 Stearidonic acid276.2 (ions) -
-
-
0.013		 0.01
0.013
0.013
0.013		 0.013
0.017
0.015
0.01		 0.015
0.01
0.013
0.01		 0.02
0.013
0.01
0.01		 0.02
0.013
0.01
0.01		 0.01
0.013
0.01
0.01		 0.015
0.01
0.01
0.01		 pmol/ug DNA
  LMFA01010023 Tricosanoic acid354.3 (ions) -
-
-
0.16		 0.083
0.153
0.123
0.1		 0.14
0.135
0.1
0.12		 0.133
0.103
0.065
0.13		 0.15
0.143
0.185
0.117		 0.09
0.095
0.12
0.153		 0.137
0.08
0.133
0.11		 0.113
0.153
0.107
0.1		 pmol/ug DNA
  - DNA0.0 (ions) -
-
-
16		 16.8
16.1
16.5
15.4		 17.1
16.5
16.6
16.3		 17.1
19.4
18.8
17.4		 19.8
19.9
20.1
19		 25.7
24.4
23.8
25.1		 25.4
23.4
26.5
29.4		 22.6
23.1
27.1
34.6		 ug/sample
  - TNF0.0 (ions) -
-
-
340		 562
484
369
358		 1582
3852
374
409		 10825
22480
411
429		 72330
77672
406
537		 110126
108866
481
599		 106528
103277
535
617		 105154
103802
1430
638		 pg/ml