LMPD Database

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LMP012378

UniProt Annotations

Entry Information
Gene Namephospholipase A2, group IVB (cytosolic)
Protein Entry
UniProt IDP0C869
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=5; Name=1; IsoId=P0C869-1, O95712-1; Sequence=Displayed; Name=2; Synonyms=beta2; IsoId=P0C869-7; Sequence=VSP_039387, VSP_039389, VSP_039390; Note=Based on a naturally occurring readthrough transcript which produces a JMJD7-PLA2G4B fusion protein.; Name=3; Synonyms=beta3; IsoId=P0C869-8; Sequence=VSP_039387, VSP_039388; Note=Based on a naturally occurring readthrough transcript which produces a JMJD7-PLA2G4B fusion protein.; Name=4; IsoId=P0C869-4, O95712-4; Sequence=VSP_019871; Note=No experimental confirmation available.; Name=5; Synonyms=Beta1; IsoId=P0C869-6; Sequence=VSP_039387; Note=Based on a naturally occurring readthrough transcript which produces a JMJD7-PLA2G4B fusion protein.;
Biophysicochemical PropertiesKinetic parameters: Vmax=7.9 pmol/min/mg enzyme (isoform 5) ; Vmax=3.6 pmol/min/ug enzyme with cAMP as substrate (isoform 4) ; Vmax=140 nmol/min/ug enzyme with 1-[14C]16:0-2-lyso-PC as substrate (isoform 5) ; Vmax=1.6 nmol/min/ug enzyme with 1-[14C]16:0-2-lyso-PC as substrate (isoform 3) ; Vmax=2.1 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PC as substrate (isoform 5) ; Vmax=0.6 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PE as substrate (isoform 5) ; Vmax=0.8 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PE as substrate (isoform 3) ; Vmax=0.3 nmol/min/ug enzyme with 1-16:0-2-[14C]18:2-PE as substrate (isoform 3) ;
Catalytic ActivityPhosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate. {ECO:0000269|PubMed:10085124, ECO:0000269|PubMed:10358058, ECO:0000269|PubMed:16617059}.
CautionMost tissues also express read-through transcripts from this gene into the upstream gene (JMJD7), some of which may encode fusion proteins
DomainThe N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+) (By similarity)
Enzyme RegulationStimulated by cytosolic Ca(2+)
FunctionCalcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine. {ECO:0000269|PubMed:10085124, ECO:0000269|PubMed:10358058, ECO:0000269|PubMed:16617059}.
Sequence CautionSequence=BAD92387.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ;
SimilarityContains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
SimilarityContains 1 PLA2c domain. {ECO:0000255|PROSITE- ProRule:PRU00555}.
Subcellular LocationCytoplasm, cytosol
Subcellular LocationIsoform 3: Mitochondrion membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Note=Translocates to membrane vesicles in a calcium-dependent fashion.
Tissue SpecificityWidely expressed. Expressed at higher level in brain, heart, liver, cerebellum and pancreas. Isoform 3 is widely expressed. {ECO:0000269|PubMed:10085124, ECO:0000269|PubMed:10358058, ECO:0000269|PubMed:16617059}.