Bone marrow-derived macrophages were obtained from tibias and femurs of mice as per protocol PP0000004702 (Harvesting and Plating Thioglycollate Elicited Macrophages for LM Distribution, pdf). Cells treatments consisted of a) Control (no treatment); b) treatment with 25-OH-cholesterol at time 0; c) lipid-depleted media containing compactin; d) treatment with 25-OH-cholesterol at time 0 in lipid-depleted media containing compactin.
Data normalization was performed as described in J Mol Endocrinol Sasik et al. 33 (1): 1 (PDF)
45,214 spots on the array
41,034 data spots
38,838 unique probes (6428 probes have no gene id)
21,609 unique mouse genes
Each gene was represented by 1 - 9 probes
Each probe was spotted 1 or 10 time