Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo2-Lipid A and ATP

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Averages of biological replicates - (GP) Glycerophospholipids

Legend:graph_icon: View data/graphs for replicatesgraph_icon: View time course graphs for averages of biological replicates
PA | PC | PE | PG | PI | PS | DNA

DetailsTime course graph
(ave of biol. reps) 		LM_ID NameMass
(neutral)		 Kdo+ATP
ATP
Kdo
Ctrl
 0 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 15 min		 Kdo+ATP
ATP
Kdo
Ctrl
 30 min		 Kdo+ATP
ATP
Kdo
Ctrl
 1 hr		 Kdo+ATP
ATP
Kdo
Ctrl
 2 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 4 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 8 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 20 hrs
Units
. . . .
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPC(16:0)495.3 (ions) -
-
-
-		 1.58
1.34
1.14
1.18		 1.71
1.13
0.378
0.802		 1.62
1.68
1.28
1.57		 1.68
2.07
1.64
2.01		 1.73
1.25
1.61
1.37		 2.6
2.12
1.82
1.78		 2.96
2.44
2.95
3.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPC(18:0)523.4 (ions) -
-
-
-		 0.33
-
0.518
1.07		 1.42
1.66
0.505
0.238		 1.27
1.11
0.9
0.971		 1.35
2.11
0.903
2.46		 0.695
0.966
1.08
1.05		 2.31
1.5
1.36
1.76		 2.04
1.05
1.34
2.21		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(28:0)677.5 (ions) -
-
-
1.92		 2.06
5.04
1.24
2.02		 3.77
1.48
1.16
2.58		 1.28
2.07
2.7
2.28		 2.85
5.34
0.761
0.741		 3.7
0.95
1.94
2.09		 1.93
2.75
2.28
1.08		 3.12
3.26
4.12
2.34		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(32:0)733.6 (ions) -
-
-
21.9		 29
14.4
17.7
13.4		 19.8
10.9
13.1
18.7		 8.81
12.8
17.3
14.3		 21.8
14.9
18.5
9.93		 30.7
20.3
24.8
22.8		 33.1
26.9
28.2
19.3		 25.3
31.6
39.6
23.3		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(32:1)731.5 (ions) -
-
-
56.2		 45
46.8
47.9
30.7		 47.4
21.9
25.1
32.6		 20.8
23.2
25.2
39		 26.4
21.9
27.1
25		 44.9
32.9
36.4
25.4		 36
29.3
33.1
42.2		 30.7
41.7
40.6
52.7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(32:2)729.5 (ions) -
-
-
7.15		 5.4
6.73
6.68
4.88		 4.61
3.45
4.2
4.87		 3.2
2.81
2.73
3.77		 3.37
6.98
4.48
2.94		 10.9
3.6
5.2
4.87		 4.04
4.89
3.31
4.31		 5.14
7.42
5.02
7.17		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(34:1)759.6 (ions) -
-
-
51.9		 58.8
50.5
49.7
42.5		 45.9
35.8
33.4
47.6		 28.2
27.4
38.9
37.4		 60.9
30.4
45
28		 59.4
43.1
34.1
42.7		 62.5
52.9
42.9
39.7		 57.6
72.1
74.1
86.4		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(34:2)757.6 (ions) -
-
-
22.1		 29
21.9
20.7
18.2		 22.6
16.6
15
21.4		 11.9
12.4
16
16.7		 25.9
18
18.4
12.4		 25.2
17.2
18.3
19.1		 24.1
23.1
14.3
16.6		 21.1
28.1
27.3
33.9		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(34:3)755.5 (ions) -
-
-
5.05		 1.71
1.19
2.43
2.54		 1.35
1.29
1.25
1.69		 1.35
1.03
1.04
2.58		 6.34
1.38
1.74
2.09		 2.14
1.39
1.27
1.15		 1.77
1.35
1.32
3.2		 9.47
2.04
4.69
1.73		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:0)789.6 (ions) -
-
-
2.89		 21.5
9.61
7.14
2.28		 15.1
1.77
1.13
1.02		 0.933
0.924
5.44
2.78		 2.13
3.28
1.06
0.46		 6.26
5.07
3.98
2.68		 1.72
2.13
3.72
4.06		 9.31
9.64
7.63
18.3		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:1)787.6 (ions) -
-
-
32.5		 33.5
29
39.2
32.8		 32.7
22.6
27.2
33.4		 17.2
20.8
27.5
33.3		 32.6
25.3
34.9
18.5		 44.9
29.2
34.5
30.1		 52.7
44.6
40.1
38.4		 60.5
75
88.2
89.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:2)785.6 (ions) -
-
-
96		 103
73.8
93.6
84.2		 82.9
62.9
56.6
83.1		 50.1
45.3
69.9
70.2		 99.5
65.1
94
57.5		 96.6
75
76.7
76.4		 112
76.8
70.6
81.1		 105
127
146
217		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:2e)/PC(36:1p)771.6 (ions) -
-
-
7.65		 7.96
12.3
11.3
7.59		 8.83
5.02
6.12
8.36		 6.22
6.88
6.22
7.53		 12
6.57
7.88
5.35		 8.36
7
8.82
9.55		 10.9
10.4
7.49
9.28		 14.9
15.7
14.7
27.4		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:3)783.6 (ions) -
-
-
20.3		 19.4
18.7
23
19.1		 20.7
16.1
13.7
19.1		 14
14.1
15.6
21		 16.4
15.1
14.5
13.4		 28.6
16.9
12.1
11.1		 28.7
21.3
19.6
21.5		 34.3
35.8
29
52.7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:3e)/PC(36:2p)769.6 (ions) -
-
-
3.7		 8.25
7.85
6.12
3.33		 4.73
3.45
2.4
4.4		 3.12
2.71
2.84
4.29		 8.12
3.8
2.91
2.09		 10
2.69
4.32
3.44		 4.25
3.84
3.1
4.48		 8.5
7.84
6.55
14.4		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:4)781.6 (ions) -
-
-
15.7		 16.4
16.2
16.7
13.4		 17.4
12.2
9.85
11.8		 11.2
10.4
10.6
14.1		 21.9
11.1
14
9.75		 25
13.7
11.4
12.3		 26.5
16.8
15.2
14.9		 23.8
27.1
23.8
53.4		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:4e)/PC(36:3p)767.6 (ions) -
-
-
5.22		 4.9
9.38
9.29
5.35		 6.1
3.02
3.1
6.76		 4.9
3.86
4.06
5.77		 11.3
8.96
4.45
3.23		 7.47
3.62
2.98
3.88		 8.54
4.76
2.96
4.41		 12
8.31
5.46
21.2		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(36:5e)/PC(36:4p)765.6 (ions) -
-
-
12.4		 13.8
7.78
8.11
7.25		 3.73
3.9
6.07
5.71		 9.17
2.93
1.9
3.17		 7.1
3.84
3.66
2.61		 11.4
5.61
9.11
4.46		 16.4
12.3
8.71
9.06		 18
12.1
23.6
12.9		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(38:3)811.6 (ions) -
-
-
14.8		 20.1
16.6
11.3
8.84		 19.5
4.66
8.82
13.1		 8.35
13.1
15.7
9.93		 9.89
14.7
10.2
6.09		 15.6
7.19
11.4
6.53		 7.36
8.92
7.32
13.6		 20.8
24.9
20.4
40.3		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(38:4)809.6 (ions) -
-
-
24.2		 14.7
12.2
20.5
15.5		 16.6
10.1
11.4
17.5		 13
11.7
13.6
19.6		 12.4
12.3
14.4
10.3		 29.4
13.3
15.9
11.1		 31.3
19.9
19
20.2		 37.5
42.5
30.9
73.2		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(38:5)807.6 (ions) -
-
-
14.9		 13.3
12.3
16.9
13.8		 19.4
10.8
11.5
14.2		 12.5
12.4
15.6
17.5		 13.1
9.96
15.7
8.24		 24.2
12.9
16.7
12.6		 26.2
19
15.9
23.2		 34.5
37.1
29.3
59.1		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(38:5e)/PC(38:4p)793.6 (ions) -
-
-
12.4		 13.2
17.1
11
7.58		 15.9
4.94
10.2
11.8		 8.69
11.1
6.47
9.29		 8.48
7.89
8.03
6.17		 17.5
10
11.6
7.87		 17.8
13.5
12.6
10.9		 16.7
24
22.3
28.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(38:6)805.6 (ions) -
-
-
23.9		 29.1
26.2
26.7
20.2		 30.8
16.6
19.1
21.8		 22.6
21.5
25.8
23.6		 31.8
25.7
27.3
16.6		 36.6
25.4
22.7
21.5		 37.7
33.1
22.8
23.3		 49.4
63
53.1
77.6		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(38:6e)/PC(38:5p)791.6 (ions) -
-
-
29.1		 10.4
12.1
22.1
7.46		 12.6
6.32
6.2
5.93		 9.72
6.2
3.96
7.62		 13
7.65
9.3
8.93		 21.4
14.3
12.9
11		 17.4
15.2
14.4
18.4		 19
33
27.4
37.7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(40:4)837.6 (ions) -
-
-
11.9		 4.33
15
8.5
5.77		 10.9
5.43
3.86
5.65		 8.89
2.7
4.24
5.29		 13.6
19
5.76
4.15		 9.92
6.62
10.7
3.78		 8.32
8.62
5.71
7.94		 17.7
17.1
16.7
29.9		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(40:5)835.6 (ions) -
-
-
16.6		 8.44
8.15
9.34
8.84		 13.6
8.69
8.96
13		 12.3
6.37
9.77
10.5		 18.6
15.5
11.5
6.19		 15.4
7.91
17.6
7.25		 20.1
11.9
11
12.7		 31.4
26.8
30.2
43.8		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PC(40:6)833.6 (ions) -
-
-
23.6		 28.9
27.2
23.3
16.1		 22.3
10.6
18.9
19.4		 20.8
13.7
21.1
27.6		 19.1
18.5
14.4
11		 35.1
18.5
16.5
21		 29.8
21.8
22.9
29.9		 43.9
69.6
51.5
82		 pmol/ug DNA