Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo2-Lipid A and ATP

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Averages of biological replicates - (GP) Glycerophospholipids

Legend:graph_icon: View data/graphs for replicatesgraph_icon: View time course graphs for averages of biological replicates
PA | PC | PE | PG | PI | PS | DNA

DetailsTime course graph
(ave of biol. reps) 		LM_ID NameMass
(neutral)		 Kdo+ATP
ATP
Kdo
Ctrl
 0 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 15 min		 Kdo+ATP
ATP
Kdo
Ctrl
 30 min		 Kdo+ATP
ATP
Kdo
Ctrl
 1 hr		 Kdo+ATP
ATP
Kdo
Ctrl
 2 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 4 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 8 hrs		 Kdo+ATP
ATP
Kdo
Ctrl
 20 hrs
Units
. . . .
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPE(16:0)453.3 (ions) -
-
-
3.27		 2.48
1.3
1.27
2.54		 1.65
1.7
1.62
3.02		 0.834
1.06
1.4
1.34		 1.11
1.54
1.98
1.08		 1.28
0.768
1.08
0.763		 0.702
0.692
0.816
0.939		 0.925
0.78
0.693
0.853		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPE(18:0)481.3 (ions) -
-
-
1.93		 3.64
2.56
2.57
2.1		 2.97
2.69
2.28
3.08		 2.39
3.62
3.77
2.43		 2.44
3.64
2.38
2.64		 2.6
2.63
2.7
2.52		 3.01
2.52
2.59
3.24		 3.36
2.37
3.95
3.61		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPE(18:1)479.3 (ions) -
-
-
11.5		 3.44
4.13
4.93
6.36		 6.98
4.77
5.21
4.94		 4.03
5.82
4.04
3.9		 6.01
4.54
4.17
2.44		 5.54
3.34
4.73
3.19		 3.1
3.47
2.77
3.5		 5.34
3.61
3.44
4.62		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPE(20:4)501.3 (ions) -
-
-
10.9		 2.59
3.58
4.32
4.7		 7.16
4.47
5.32
5.24		 3.46
4.81
4.34
4.11		 6.26
5.51
3.53
2.63		 4.5
3.3
7.11
3.55		 4.45
3.77
2.78
3.86		 5.68
4.42
4.36
4.98		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPE(22:5)527.3 (ions) -
-
-
4.35		 1.72
2.03
2.18
2.92		 3.57
2.27
3.02
1.83		 1.91
3.18
2.03
2.22		 3.08
2.65
1.97
1.59		 2.14
1.56
3.6
2.12		 2.65
2.19
1.99
2.38		 3.73
2.44
2.43
3.18		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- LPE(22:6)525.3 (ions) -
-
-
6.99		 1.38
1.69
1.86
2.14		 2.81
1.94
2.34
2.62		 1.38
2.84
2.21
2.31		 2.14
2.2
1.56
1.08		 2.23
1.3
3.2
1.9		 1.94
2
1.99
1.89		 2.74
2.1
2.16
2.39		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(32:0)691.5 (ions) -
-
-
2.75		 1.15
2.08
1.69
1.07		 3.09
0.663
1.03
1.22		 1.77
1.93
2.64
2.11		 3.08
3.38
1.29
0.623		 3.61
2.12
2.07
1.79		 1.18
2.11
2.15
1.86		 4.03
2.64
2.56
1.81		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(32:1)689.5 (ions) -
-
-
4.82		 4.25
5.45
3.62
3.31		 6.04
2.92
3.35
3.42		 4.46
4.4
5.1
4.5		 4.25
3.58
3.59
1.89		 5.45
4.28
5.46
4.12		 3.74
3.18
5.92
3.75		 7.35
6.44
6.99
4.52		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(34:0)719.5 (ions) -
-
-
4.56		 1.55
2.01
1.46
1.7		 2.89
1.23
2.38
1.93		 4.11
3.5
4.22
2.56		 5.31
2.06
1.22
1.34		 1.66
2.28
5.24
1.99		 2.58
1.33
2.59
3.34		 3.11
2.99
4.69
1.43		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(34:1)717.5 (ions) -
-
-
18.9		 18.5
17.8
12.7
17.4		 15.3
11.4
16.5
14.1		 20.2
17.4
18.7
17.1		 21.7
13
19.6
12		 20.7
15.3
23.2
17.4		 21
17.7
20.5
17.4		 23.4
22.5
33.7
24.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(34:2)715.5 (ions) -
-
-
10.2		 8.66
9.48
5.98
7.15		 8.76
5.02
6.77
6.57		 9.17
8.76
9.92
6.96		 11.2
6.83
7.83
5.06		 11.5
9.37
8.38
6.83		 7.03
6.25
7.48
5.98		 7.59
11.2
11.7
8.69		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:0)747.6 (ions) -
-
-
7.85		 12.4
10.4
11.8
12.7		 9.65
8.8
12.3
11.2		 12.5
11.5
13.6
12.7		 9.65
10.1
13.2
9.02		 11.6
9.81
7.74
10.8		 14.8
13.2
13.5
12		 14.1
13.1
14.7
13.6		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:1)745.6 (ions) -
-
-
34.6		 29.2
32.1
28.2
32.6		 34.2
24.9
37.7
25.5		 42.4
36.8
39.7
34.2		 42.3
32.1
39.6
28.2		 43
31
42.5
33.6		 46.6
45.3
41.1
36.2		 43.4
41.8
51.6
46.7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:1e)/PE(36:0p)731.6 (ions) -
-
-
2.82		 3.11
3.09
3.67
3.06		 4.43
2.34
3.74
1.43		 4.89
3.45
4.13
3.9		 5.73
2.47
2.1
2.57		 3.09
4.64
3.56
2.81		 4.32
4.69
3.98
3.98		 3.46
2.81
3.22
4.04		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:2)743.5 (ions) -
-
-
42.3		 37.7
44.6
34.1
42		 40.8
27.9
45.5
32.7		 48.3
44.2
45.2
40.2		 49.2
35.3
49.2
33.6		 46.5
34.8
49.8
39.6		 44.1
42.3
41
40		 39.7
44.2
49.4
52.6		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:2e)/PE(36:1p)729.6 (ions) -
-
-
9.1		 18.5
16.8
15.3
15		 13.6
11.9
14
15.2		 17.7
11.1
18.2
15.8		 10.9
14.7
14.7
10		 14.1
13.1
9.26
15.1		 18.9
19.8
16.7
14.7		 18.6
18.4
16.5
17.2		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:3)741.5 (ions) -
-
-
9.01		 11.6
9.78
7.34
9.68		 8.37
5.13
9.61
8.29		 12.4
7.89
8.69
9.53		 10.1
6.81
9.25
6.94		 9.68
7.68
12.9
7.87		 11.4
9.62
9.12
8.7		 13
11
13
11.9		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:3e)/PE(36:2p)727.6 (ions) -
-
-
7.24		 12
19
10.1
10.8		 12.6
11.4
10.8
11.1		 6.23
8.05
11.3
11.9		 13.8
9.27
10
11.3		 10.5
8.47
8.08
6.86		 11.7
12
11.2
9.91		 10.7
11.5
9.81
11.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:4)739.5 (ions) -
-
-
9.91		 9.07
10.3
6.57
8.37		 9.01
6.15
8.45
7.12		 8.57
7.1
8.49
8.97		 9.64
7.57
9.98
7.46		 10.5
8.51
12.2
7.48		 9.18
9.96
8.65
8.8		 9.27
12.6
15
11.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:4e)/PE(36:3p)725.5 (ions) -
-
-
5.67		 11.7
10.7
8.86
9.22		 8.62
7.39
8.25
7.94		 8.71
7.25
10.8
10.1		 12.8
7.99
9.08
6.97		 8.87
7.76
6.65
5.42		 10.7
9.88
9.79
8.69		 10.7
9.96
10.6
10.6		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(36:5e)/PE(36:4p)723.5 (ions) -
-
-
14.4		 24.3
21.5
17.5
20.6		 18.6
16.8
18.5
19.2		 25.4
18.2
23.2
22.2		 15.2
20.4
22.5
13.8		 21.7
17.6
12.3
19.2		 25.9
23.6
20.7
17		 29.8
23.1
23.7
25.8		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:3)769.6 (ions) -
-
-
16.7		 9.66
16.8
17.2
17.1		 14.8
18.3
16.2
12		 18.2
17.2
20
14.9		 20.7
16.1
12.1
19.1		 17.8
14.9
20
16.1		 23.9
23.4
21.7
17.2		 23.3
19.5
23
22.2		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:3e)/PE(38:2p)755.6 (ions) -
-
-
7.37		 10.1
10.8
10.7
11.7		 11.4
9.61
9.1
7.38		 11.8
5.49
12.1
11.3		 10.3
8.76
6.13
9.22		 11.5
9.87
9.89
10.1		 12.6
9.16
10.2
11		 11.7
10.9
9.49
11.8		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:4)767.5 (ions) -
-
-
32.7		 32.4
31.4
35
36.9		 36.3
27.1
39.1
26.8		 37.5
33.9
42.5
35.3		 42.2
37.4
38.2
29.9		 38.6
32.8
42.8
35.9		 45.4
47.1
40.8
37.1		 44.2
39.5
51.8
47.7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:5)765.5 (ions) -
-
-
13		 15.8
13.5
14.6
18.1		 15.4
12.3
18
11.8		 16.3
16.8
16.2
17		 20.1
14.8
18
13.9		 18
14.4
20.5
15.7		 19.2
19.1
17.7
17		 15.9
17.3
23.8
22.7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:5e)/PE(38:4p)751.6 (ions) -
-
-
15		 30.5
30.6
25.8
27.4		 23.2
19.7
21.5
28.1		 27.4
14.9
30.2
22.5		 17.7
19.8
23.8
20.1		 19.7
20.1
16.4
24.4		 30.5
25.1
27.5
24.5		 33.3
27.2
29.6
25.5		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:6)763.5 (ions) -
-
-
5.64		 4.18
4.85
4.6
5.92		 5.69
3.87
5.61
3.96		 8.43
4.12
6
7.18		 7.16
4.87
5.69
4.2		 8.62
5.67
6.52
4.48		 7.78
4.96
7.33
6.39		 8.25
8.51
7.23
7		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(38:6e)/PE(38:5p)749.5 (ions) -
-
-
19.5		 24.7
25.5
21.6
24.1		 20.6
15.3
14.5
21		 21
15.6
24.5
24.5		 14.6
13.6
15
17.5		 17.2
18.2
14.1
13.8		 24.4
21.5
21.5
20		 27.3
21.1
21.8
23.8		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(40:4)795.6 (ions) -
-
-
5.08		 4.43
5.11
5.27
5.11		 5.29
3.61
5.7
3.75		 7.22
10.4
7.76
4.61		 6.25
5.05
5.5
4.91		 5.25
4.46
6.45
5.3		 8.2
8.02
6.75
5.28		 7.04
5.73
7.58
7.03		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(40:5)793.6 (ions) -
-
-
8.34		 7.83
8.34
9.61
10.1		 9.65
6.69
10.4
6.93		 10.1
7.59
10.3
8.64		 9.04
8.61
10.1
8.6		 9.54
6.86
11.1
9.51		 13.7
13.7
12.2
10.2		 15.2
10.8
12.5
13.1		 pmol/ug DNA
Legend: :Ctrl  :Kdo2-lipid A  :ATP  :ATP+Kdo2-lipid A
- PE(40:6)791.5 (ions) -
-
-
14.2		 9.91
9.13
11.7
12.5		 11.7
8.45
13.1
9.04		 12.1
10.3
12.3
10.5		 12.6
10.6
12.9
10.1		 12.5
9.71
13.2
11		 16.1
16.2
13.6
11.9		 16.1
12.5
15.8
14.7		 pmol/ug DNA