Mass Spectrometry of Fatty Acid Derivatives
These pages are tutorials that deal with the application of mass spectrometry (MS) with electron-impact ionization and linked to gas chromatography (GC) for the determination of the structures of fatty acids. They tend to be largely practical as opposed to mechanistic accounts. Although methyl esters are used almost universally for GC separations and often for MS, their mass spectra afford limited structural information only. On the other hand, certain nitrogen-containing derivatives, especially pyrrolidides, 4,4‑dimethyloxazoline (DMOX) derivatives and 3‑pyridylcarbinol ('picolinyl') esters, give much more informative data as discussed in the attached web pages in which more than 735 mass spectra are illustrated (not counting the Archive sections with these and a further 1500 spectra). Spectra of some selected other lipids are also discussed but only those encountered in our research efforts and for which we have spectra. Whenever new information and mass spectra become available, these web pages will be updated.
If you are new to the subject, you may wish to first consult -
In the following section, web pages describe the virtues of the different types of derivative together with practical methods for their preparation. Some ancillary practical techniques and other methods that aid analysis are also discussed.
Other Main Topics
Documents are grouped into five main sections according to the nature of the derivatives used. Mass spectra of most of the important fatty acids of animals and plants are described, together with some less usual, substituted fatty acids. They can be addressed from the following links -
Archives of Mass Spectra - All our mass spectra (2232 in total) are available here for study or reference, but without interpretation, in Archives of methyl esters - 3-pyridylcarbinol esters - 4,4'-dimethyloxazolines - pyrrolidides - alkyl esters - other lipids.
Recommended Book: Christie, W.W. and Han, X. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (4th edition), 446 pages (Oily Press, Woodhead Publishing and now Elsevier) (2010) - at Science Direct.
|© Author: William W. Christie
|Updated: November 8th, 2023