LMPD Database

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LMP007679

UniProt Annotations

Entry Information
Gene Name2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Protein EntryMENH_ECOLI
UniProt IDP37355
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=10.1 uM for SEPHCHC (at 25 degrees Celsius and pH 7) {ECO:0000269|PubMed:18284213};
Catalytic Activity5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3- ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4- diene-1-carboxylate + pyruvate. {ECO:0000255|HAMAP-Rule:MF_01660, ECO:0000269|PubMed:18284213}.
FunctionCatalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro. {ECO:0000269|PubMed:15808744, ECO:0000269|PubMed:18284213}.
PathwayQuinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 3/7. {ECO:0000255|HAMAP-Rule:MF_01660}.
PathwayQuinol/quinone metabolism; menaquinone biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01660}.
Sequence CautionSequence=AAA24151.1; Type=Frameshift; Positions=182; Evidence={ECO:0000305}; Sequence=M93421; Type=Frameshift; Positions=182; Evidence={ECO:0000305};
SimilarityBelongs to the AB hydrolase superfamily. MenH family. {ECO:0000255|HAMAP-Rule:MF_01660, ECO:0000305}.
SubunitMonomer. {ECO:0000255|HAMAP-Rule:MF_01660, ECO:0000269|PubMed:18284213}.