LMPD Database

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LMP007681

UniProt Annotations

Entry Information
Gene Nameweak acylphosphatase
Protein EntryACYP_ECOLI
UniProt IDP0AB65
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.54 mM for benzoylphosphate {ECO:0000269|PubMed:17134700}; pH dependence: Optimum pH is 5.2-6.5. {ECO:0000269|PubMed:17134700}; Temperature dependence: Thermostable. Retains 85-90 % of its activity after 3 hours of incubation at 90 degrees Celsius. {ECO:0000269|PubMed:17134700};
Catalytic ActivityAn acylphosphate + H(2)O = a carboxylate + phosphate. {ECO:0000255|HAMAP-Rule:MF_01450, ECO:0000269|PubMed:17134700}.
MiscellaneousHas a considerably reduced catalytic efficiency compared to other mesophilic acylphosphatases. Shows a considerable resistance against urea denaturation since the full enzymatic activity is maintained in the presence of urea concentrations approaching 6.0 M and that only a slight decreae of 10-15 % was observed with higher urea concentrations.
SimilarityBelongs to the acylphosphatase family. {ECO:0000255|HAMAP-Rule:MF_01450}.
SimilarityContains 1 acylphosphatase-like domain. {ECO:0000255|HAMAP-Rule:MF_01450}.