LMPD Database

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LMP007707

UniProt Annotations

Entry Information
Gene Name2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase; SEPHCHC synthase
Protein EntryMEND_ECOLI
UniProt IDP17109
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=53 nM for isochorismate (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; KM=1.5 uM for 2-oxoglutarate (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; KM=2.4 uM for thiamine diphosphate (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; KM=80 uM for magnesium ions (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; pH dependence: Optimum pH is 7-8. {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421};
Catalytic ActivityIsochorismate + 2-oxoglutarate = 5- enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO(2). {ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000269|PubMed:17760421}.
CautionWas originally thought (PubMed:1459959, PubMed:14621995 and PubMed:16511076) to be a 2-succinyl-6-hydroxy-2,4- cyclohexadiene-1-carboxylate (SHCHC) synthase but further protein analysis clearly suggests that it is a 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) synthase. {ECO:0000305|PubMed:17760421}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01659}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_01659};
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000255|HAMAP-Rule:MF_01659}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000255|HAMAP-Rule:MF_01659};
FunctionCatalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate (SEPHCHC). {ECO:0000255|HAMAP- Rule:MF_01659, ECO:0000269|PubMed:17760421}.
InductionBy isopropyl-beta-D-thiogalactoside (IPTG). {ECO:0000269|PubMed:1459959}.
PathwayQuinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 2/7. {ECO:0000255|HAMAP-Rule:MF_01659}.
PathwayQuinol/quinone metabolism; menaquinone biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01659}.
Sequence CautionSequence=AAA24153.1; Type=Frameshift; Positions=370; Evidence={ECO:0000305};
SimilarityBelongs to the TPP enzyme family. MenD subfamily. {ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000305}.
SubunitHomodimer. {ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:16511076}.