LMPD Database

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LMP007714

UniProt Annotations

Entry Information
Gene NameUDP-2,3-diacylglucosamine pyrophosphohydrolase
Protein EntryLPXH_ECOLI
UniProt IDP43341
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=61.7 uM for UDP-2,3-bis((3R)-3-hydroxymyristoyl)-alpha-D- glucosamine {ECO:0000269|PubMed:12000770}; Vmax=17.2 umol/min/mg enzyme {ECO:0000269|PubMed:12000770}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:12000770};
Catalytic ActivityUDP-2,3-bis((3R)-3-hydroxymyristoyl)-alpha-D- glucosamine + H(2)O = 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D- glucosaminyl 1-phosphate + UMP. {ECO:0000255|HAMAP-Rule:MF_00575, ECO:0000269|PubMed:12000770, ECO:0000269|PubMed:12000771}.
Enzyme RegulationInhibited by Triton X-100.
FunctionCatalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1- phosphate (lipid X) and UMP.
PathwayGlycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 4/6. {ECO:0000255|HAMAP- Rule:MF_00575}.
SimilarityBelongs to the LpxH family. {ECO:0000255|HAMAP- Rule:MF_00575}.
Subcellular LocationCytoplasm.