LMPD Database

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LMP007721

UniProt Annotations

Entry Information
Gene Nameenoyl-[acyl-carrier-protein] reductase, NADH-dependent
Protein EntryFABI_ECOLI
UniProt IDP0AEK4
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=3.3 uM for trans-2-dodecenoyl-ACP (DD-ACP)(at 25 degrees Celsius and pH 8) {ECO:0000269|PubMed:17012233, ECO:0000269|PubMed:8119879}; KM=22 uM for crotonyl-ACP (at 30 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17012233, ECO:0000269|PubMed:8119879}; KM=24 uM for trans-2-dodecenoyl-CoA (DD-CoA)(at 25 degrees Celsius and pH 8) {ECO:0000269|PubMed:17012233, ECO:0000269|PubMed:8119879}; KM=2700 uM for crotonyl-CoA (at 30 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17012233, ECO:0000269|PubMed:8119879};
Catalytic ActivityAn acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH. {ECO:0000269|PubMed:8119879}.
Enzyme RegulationInhibited by diazaborines, triclosan (5-chloro- 2-2,4-dichlorophenoxyphenol), 1,4-disubstituted imidazoles, 1,4- benzodiazepine derivatives, naphthyridinone derivatives, luteolin and curcumin. {ECO:0000269|PubMed:10398587, ECO:0000269|PubMed:10493822, ECO:0000269|PubMed:11514139, ECO:0000269|PubMed:12109908, ECO:0000269|PubMed:12699381, ECO:0000269|PubMed:19959361, ECO:0000269|PubMed:8119879, ECO:0000269|PubMed:8953047, ECO:0000269|PubMed:9707111}.
FunctionCatalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis. {ECO:0000269|PubMed:20693992, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8119879}.
MiscellaneousThe antibiotic diazaborine interferes with the activity by binding to the protein and NAD.
PathwayCofactor biosynthesis; biotin biosynthesis.
PathwayLipid metabolism; fatty acid biosynthesis.
SimilarityBelongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily. {ECO:0000305}.
SubunitHomotetramer. {ECO:0000269|PubMed:10201369, ECO:0000269|PubMed:10398587, ECO:0000269|PubMed:10493822, ECO:0000269|PubMed:10595560, ECO:0000269|PubMed:11514139, ECO:0000269|PubMed:12109908, ECO:0000269|PubMed:12699381, ECO:0000269|PubMed:8953047}.