LMPD Database

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LMP007724

UniProt Annotations

Entry Information
Gene Namebifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase
Protein EntryFCL_ECOLI
UniProt IDP32055
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=9 uM for NADPH {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; KM=109 uM for NADH {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; KM=29 uM for GDP-4-keto-6-deoxymannose {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; Vmax=363.5 umol/min/mg enzyme {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; pH dependence: Optimum pH is 6-6.5. {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971};
Catalytic ActivityGDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-6- deoxy-alpha-D-mannose + NADPH. {ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9473059}.
Enzyme RegulationSubject to product inhibition by NADP and GDP- fucose. {ECO:0000269|PubMed:10480878}.
FunctionCatalyzes the two-step NADP-dependent conversion of GDP- 4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. {ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9473059}.
PathwayExopolysaccharide biosynthesis; colanic acid biosynthesis. {ECO:0000269|PubMed:9473059}.
PathwayNucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2. {ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:9473059}.
SimilarityBelongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily. {ECO:0000255|HAMAP- Rule:MF_00956}.
Subcellular LocationCytoplasm.
SubunitHomodimer. {ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9817848, ECO:0000269|PubMed:9862812}.