LMPD Database

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LMP007734

UniProt Annotations

Entry Information
Gene Nameacetyl-CoA synthetase
Protein EntryACSA_ECOLI
UniProt IDP27550
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.15 mM for ATP (at pH 8.5 and 37 degrees Celsius) {ECO:0000269|PubMed:21941}; KM=0.2 mM for CoA (at pH 8.5 and 37 degrees Celsius) {ECO:0000269|PubMed:21941}; KM=0.2 mM for acetate (at pH 8.5 and 37 degrees Celsius) {ECO:0000269|PubMed:21941}; Vmax=100 nmol/min/mg enzyme (at pH 8.5 and 37 degrees Celsius) {ECO:0000269|PubMed:21941}; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:21941};
Catalytic ActivityATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. {ECO:0000255|HAMAP-Rule:MF_01123, ECO:0000269|PubMed:21941, ECO:0000269|PubMed:7751300}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01123, ECO:0000269|PubMed:21941};
Disruption PhenotypeCells lacking this gene grow as well as wild-type cells at high concentrations (>25 mM) of acetate as the sole carbon source, but grow poorly on lower concentrations (<10 mM). {ECO:0000269|PubMed:7751300}.
FunctionCatalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. Acs undergoes a two-step reaction. In the first half reaction, Acs combines acetate with ATP to form acetyl- adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.
FunctionEnables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates CheY, the response regulator involved in flagellar movement and chemotaxis.
InductionBy CRP and FNR, in response to rising cAMP levels, falling oxygen partial pressure and changes in carbon flux. May also be induced by acetate. {ECO:0000269|PubMed:10894724}.
PtmAutoacetylated. Deacetylation by CobB activates the enzyme.
Sequence CautionSequence=AAA24715.1; Type=Frameshift; Positions=323; Evidence={ECO:0000305};
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000255|HAMAP-Rule:MF_01123}.
SubunitForms a 1:1 complex with CobB/NAD-dependent deacetylase.