LMPD Database

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LMP009742

UniProt Annotations

Entry Information
Gene NameABC transporter C family member 2
Protein EntryAB2C_ARATH
UniProt IDQ42093
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=750 uM for E(2)17betaG (at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=240 uM for E(2)17betaG (with 100 uM DNP-GS at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=4.7 uM for E(2)17betaG (with 500 uM GSH at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=66 uM for DNP-GS (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=20 uM for DNP-GS (with 100 uM E(2)17betaG at pH 8 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=73 uM for GSSG (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=75 uM for MOC-GS (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; KM=15 uM for Bn-NCC-1 (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=8.8 nmol/min/mg enzyme with E(2)17betaG as substrate (at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=11.5 nmol/min/mg enzyme with E(2)17betaG as substrate (with 100 uM DNP-GS at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=33 nmol/min/mg enzyme with E(2)17betaG as substrate (with 500 uM GSH at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=2 nmol/min/mg enzyme with DNP-GS as substrate (at pH 8 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=1.8 nmol/min/mg enzyme with DNP-GS as substrate (with 100 uM E(2)17betaG at pH 8 and 25 degrees Celsius) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=3.8 nmol/min/mg enzyme with GSSG as substrate (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=13.6 nmol/min/mg enzyme with MOC-GS as substrate (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}; Vmax=6.3 nmol/min/mg enzyme with Bn-NCC-1 as substrate (at pH 8) {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749};
Catalytic ActivityATP + H(2)O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Enzyme RegulationReciprocal promotion of DNP-GS and E(2)17betaG uptake. E(2)17betaG uptake is also stimulated by GSH and S-methyl- glutathione (S-methyl-GS), and, to a lower extent, by GSSG and C3G-GS. Metolachlor-GS and decyl-GS slightly inhibit E(2)17betaG uptake. {ECO:0000269|PubMed:11115509}.
FunctionPump for glutathione S-conjugates. Mediates the transport of S-conjugates such as GSH, S-(2,4-dinitrophenyl)- glutathione (DNP-GS), GSSG, cyanidin 3-glucoside-GS (C3G-GS) and metolachlor-GS (MOC-GS), glucuronides such as 17-beta-estradiol 17-(beta-D-glucuronide) (E(2)17betaG), and of the chlorophyll catabolite such as B.napus nonfluorescent chlorophyll catabolite (Bn-NCC-1). {ECO:0000269|PubMed:11115509, ECO:0000269|PubMed:9490749}.
InductionBy 1-chloro-2,4-dinitrobenzene (CDNB). {ECO:0000269|PubMed:9271206}.
Sequence CautionSequence=AAC04246.1; Type=Frameshift; Positions=1212, 1223; Evidence={ECO:0000305}; Sequence=AAC49798.1; Type=Frameshift; Positions=1461; Evidence={ECO:0000305}; Sequence=CAA81250.1; Type=Frameshift; Positions=1223, 1234; Evidence={ECO:0000305};
SimilarityBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. {ECO:0000305}.
SimilarityContains 2 ABC transmembrane type-1 domains. {ECO:0000255|PROSITE-ProRule:PRU00441}.
SimilarityContains 2 ABC transporter domains. {ECO:0000255|PROSITE-ProRule:PRU00434}.
Subcellular LocationVacuole membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}. Note=Tonoplast. {ECO:0000269|PubMed:11115509}.
SubunitInteracts with FKBP42/TWD1 and probably with calmodulin (CaM). {ECO:0000269|PubMed:15133126}.
Tissue SpecificityUbiquitous, at low levels. {ECO:0000269|PubMed:12430019, ECO:0000269|PubMed:9490749}.