LMPD Database

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LMP010069

UniProt Annotations

Entry Information
Gene Namecytochrome P450 710A1
Protein EntryC7101_ARATH
UniProt IDO64697
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=1 uM for beta-sisterol {ECO:0000269|PubMed:16531502};
CofactorName=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Disruption PhenotypeNo visible phenotype under normal growth condition, but upon infection with P.syringae, no accumulation of stigmasterol and increased resistance to the pathogen. {ECO:0000269|PubMed:20444228}.
FunctionRequired to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward beta- sitosterol and produces stigmasterol. {ECO:0000269|PubMed:16531502, ECO:0000269|PubMed:17909855, ECO:0000269|PubMed:20444228}.
InductionBy infection with Pseudomonas syringae. {ECO:0000269|PubMed:20444228}.
MiscellaneousPlants overexpressing CYP710A2 show higher levels of stigmasterol and lower levels of beta-sitosterol than the wild- type.
PathwaySteroid biosynthesis; sterol biosynthesis.
SimilarityBelongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular LocationMembrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Tissue SpecificityExpressed in the vascular tissues of roots, shoots and leaves. Expressed in root tips and sepals. {ECO:0000269|PubMed:16531502}.